Molecular characterization of extended-spectrum β-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey

Mariagrazia Perilli, Emanuela Dell'Amico, Bernardetta Segatore, Maria Rosaria De Massis, Ciro Bianchi, Francesco Luzzaro, Gian Maria Rossolini, Antonio Toniolo, Giuseppe Nicoletti, Gianfranco Amicosante

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Extended-spectrum β-lactamases (ESBLs) are widespread in hospital settings worldwide. The present investigation was undertaken to assess the distribution and prevalence of ESBLs belonging to the TEM and SHV families in 448 ESBL-producing clinical isolates of Enterobacteriaceae collected from 10 different Italian hospitals. The natures of TEM and SHV determinants were identified by direct sequencing of PCR-amplified genes. TEM-52 and SHV-12 were the most common variants, and they were found in most hospitals and in several different species. Other less frequent variants included TEM-5, TEM-12, TEM-15, TEM-19, TEM-20, TEM-24, TEM-26, TEM-43, TEM-60, TEM-72, TEM-87, SHV-2a, SHV-5, and SHV-11. Proteus mirabilis was the most common producer of TEM-type ESBLs, while Klebsiella pneumoniae was the most common producer of SHV-type ESBLs. The distribution of TEM- and SHV-type ESBL variants in Enterobacteriaceae from Italian hospitals exhibited notable differences from those from other geographical settings.

Original languageEnglish
Pages (from-to)611-614
Number of pages4
JournalJournal of Clinical Microbiology
Volume40
Issue number2
DOIs
Publication statusPublished - 2002

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Enterobacteriaceae
Proteus mirabilis
Klebsiella pneumoniae
Polymerase Chain Reaction
Surveys and Questionnaires
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Molecular characterization of extended-spectrum β-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey. / Perilli, Mariagrazia; Dell'Amico, Emanuela; Segatore, Bernardetta; De Massis, Maria Rosaria; Bianchi, Ciro; Luzzaro, Francesco; Rossolini, Gian Maria; Toniolo, Antonio; Nicoletti, Giuseppe; Amicosante, Gianfranco.

In: Journal of Clinical Microbiology, Vol. 40, No. 2, 2002, p. 611-614.

Research output: Contribution to journalArticle

Perilli, M, Dell'Amico, E, Segatore, B, De Massis, MR, Bianchi, C, Luzzaro, F, Rossolini, GM, Toniolo, A, Nicoletti, G & Amicosante, G 2002, 'Molecular characterization of extended-spectrum β-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey', Journal of Clinical Microbiology, vol. 40, no. 2, pp. 611-614. https://doi.org/10.1128/JCM.40.2.611-614.2002
Perilli, Mariagrazia ; Dell'Amico, Emanuela ; Segatore, Bernardetta ; De Massis, Maria Rosaria ; Bianchi, Ciro ; Luzzaro, Francesco ; Rossolini, Gian Maria ; Toniolo, Antonio ; Nicoletti, Giuseppe ; Amicosante, Gianfranco. / Molecular characterization of extended-spectrum β-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 2. pp. 611-614.
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