Molecular characterization of group C rotaviruses detected in children in Italy

Maria Cristina Medici, Laura Anna Abelli, Monica Martinelli, Vito Martella, Giuseppe Dettori, Carlo Chezzi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Group C rotavirus (GCRV) infection has been described worldwide in children and adults either as sporadic cases or large outbreaks of gastroenteritis but GCRV epidemiology is still unclear. Objective: To acquire molecular information on GCRV infection in children hospitalized with gastroenteritis in Italy. Study design: Stools positive for rotavirus-like-particles by electron microscopy during the 2004-2005 surveillance for rotavirus infection in Parma, Italy, were screened by polyacrylamide gel electrophoresis. GCRV strains detected were characterized by sequence and phylogenetic analysis of the genes encoding the capsid proteins VP4, VP6 and VP7. Results: Two of 856 samples (0.23%; 0.7% of 273 samples containing rotavirus-like particles) contained GCRV. These Italian strains were virtually identical in the 3 genes and were closely related to human strains identified in Asia, rather than to strains of European origin. Conclusions: These results confirm the circulation of GCRVs in Europe and demonstrate genetic heterogeneity among European GCRVs. Inclusion of GCRVs in the diagnostic algorithms for childhood diarrhoea could be helpful in monitoring temporal changes in the GCRV epidemiology, likely under the influence of the changing demographic dynamics.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalJournal of Clinical Virology
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Rotavirus
Italy
Rotavirus Infections
Gastroenteritis
Epidemiology
Hospitalized Child
Genetic Heterogeneity
Capsid Proteins
Genes
Disease Outbreaks
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Diarrhea
Electron Microscopy
Demography

Keywords

  • Group C rotaviruses
  • Molecular epidemiology
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Medici, M. C., Abelli, L. A., Martinelli, M., Martella, V., Dettori, G., & Chezzi, C. (2009). Molecular characterization of group C rotaviruses detected in children in Italy. Journal of Clinical Virology, 44(1), 62-65. https://doi.org/10.1016/j.jcv.2008.10.006

Molecular characterization of group C rotaviruses detected in children in Italy. / Medici, Maria Cristina; Abelli, Laura Anna; Martinelli, Monica; Martella, Vito; Dettori, Giuseppe; Chezzi, Carlo.

In: Journal of Clinical Virology, Vol. 44, No. 1, 01.2009, p. 62-65.

Research output: Contribution to journalArticle

Medici, MC, Abelli, LA, Martinelli, M, Martella, V, Dettori, G & Chezzi, C 2009, 'Molecular characterization of group C rotaviruses detected in children in Italy', Journal of Clinical Virology, vol. 44, no. 1, pp. 62-65. https://doi.org/10.1016/j.jcv.2008.10.006
Medici, Maria Cristina ; Abelli, Laura Anna ; Martinelli, Monica ; Martella, Vito ; Dettori, Giuseppe ; Chezzi, Carlo. / Molecular characterization of group C rotaviruses detected in children in Italy. In: Journal of Clinical Virology. 2009 ; Vol. 44, No. 1. pp. 62-65.
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