Molecular characterization of hospital-acquired methicillin-resistant Staphylococcus aureus strains in pediatric outbreaks using variable tandem repeat analysis with spa and ClfB typing

Elisabetta Ugolotti, Roberto Bandettini, Anna Marchese, Laura Gualco, Irene Vanni, Luana Borzi, Eddi Di Marco, Elio Castagnola, Giovanni Melioli, Roberto Biassoni

Research output: Contribution to journalArticle

Abstract

To analyze 67 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolated from pediatric hospital infections, we used multilocus variable-number tandem-repeat DNA sequence-based techniques, targeting the protein A polymorphic X region and the clumping factor B complete R domain. We define a" clfB similarity score" and then compare the double loci analysis of closely related MRSA isolates with pulsed-field gel electrophoresis (PFGE). We found an endemic clone (MLST-ST8, spa-t008, SCC. mecIV, ClfB lineage 1) able to originate 3 possible outbreaks and a second clone (MLST-ST152, spa-t355, SCC. mecV, ClfB lineage 4) responsible for limited cases of MRSA infections, indicating that the combination of spa and clfB-lineage typing is useful to trace MRSA pediatric outbreaks.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume69
Issue number2
DOIs
Publication statusPublished - Feb 2011

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Keywords

  • ClfB
  • MRSA
  • Repeat assay analysis
  • S. aureus protein A
  • Sequence typing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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