Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells

Alessandro Salvi, Italia Bongarzone, Lia Ferrari, Edoardo Abeni, Bruna Arici, Maida De Bortoli, Sabrina Scuri, Daniela Bonini, Ilaria Grossi, Ana Benetti, Gianluca Baiocchi, Nazario Portolani, Giuseppina De Petro

Research output: Contribution to journalArticle

Abstract

Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. We have previously reported that LASP-1 is a downstream protein of the urokinase type plasminogen activator (uPA). Here we investigated the role of LASP-1 in HCC by a molecular and biological characterization of LASP-1 expression in human HCC specimens and in cultured HCC cells. We determined the LASP-1 mRNA expression levels in 55 HCC cases with different hepatic background disease. We identified 3 groups of patients with high, equal or low LASP-1 mRNA levels in HCC tissues compared to the peritumoral (PT) tissues. In particular we found that i) the HCCs displayed a higher LASP-1 mRNA level in HCC compared to PT tissues; ii) the expression levels of LASP-1 mRNA in female HCCs were significantly higher compared to male HCCs; iii) the cirrhotic HCCs displayed a higher LASP-1 mRNA. Further, the biological characterization of the ectopic LASP-1 overexpression in HCC cells, using MALDI-TOF mass spectrometer on the LASP-1 co-immunoprecipitated fractions, displayed vimentin as a novel putative partner of LASP-1. Our results suggest that LASP-1 mRNA overexpression may be mainly implicated in female HCCs and cirrhotic HCCs; and that LASP1 may play its role with vimentin in HCC cells.

Original languageEnglish
Pages (from-to)1901-1912
Number of pages12
JournalInternational Journal of Oncology
Volume46
Issue number5
DOIs
Publication statusPublished - May 1 2015

Keywords

  • Hepatocellular carcinoma
  • LASP-1
  • Mass spectrometry
  • qPCR
  • Vimentin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Salvi, A., Bongarzone, I., Ferrari, L., Abeni, E., Arici, B., De Bortoli, M., Scuri, S., Bonini, D., Grossi, I., Benetti, A., Baiocchi, G., Portolani, N., & De Petro, G. (2015). Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. International Journal of Oncology, 46(5), 1901-1912. https://doi.org/10.3892/ijo.2015.2923