Molecular characterization of Ph′+ hybrid acute leukemia

Francesco Lo Coco, Giuseppe Basso, Paola Francia Di Celle, Angela Tassinari, Daniela Pasqualetti, Maria R. De Cuia, Maria C. Putti, Giovanni Del Poeta, Carola Ponzetto, Giuseppe Saglio, Robert Foa

Research output: Contribution to journalArticlepeer-review

Abstract

The configuration of the immunoglobulin heavy chain (IgH), T-cell receptor (TcR) β and γ chain regions, and the major breakpoint cluster region (M-bcr) genes were analysed in four cases of Ph′+ acute leukemia (AL). Monoclonal rearrangements of the IgH region were detected in three cases exhibiting two phenotypically distinct cell populations (i.e. one lymphoid and one myeloid). In one of these cases, identical genetic events were observed by molecular analysis of FACS separated blasts. Multi-lineage rearrangements involving also the TcR γ gene were observed in a biphenotypic AL showing co-expression of markers. The lack of rearrangements within the M-bcr gene, together with demonstration in one case of the Ph′+ AL specific p190 protein product, pointed against the occurrence of chronic myeloid leukemias presenting in blastic transformation. Our results imply that such cases are to be considered as true AL and should therefore be included in the definition of hybrid AL.

Original languageEnglish
Pages (from-to)1061-1067
Number of pages7
JournalLeukemia Research
Volume13
Issue number12
DOIs
Publication statusPublished - 1989

Keywords

  • bcr
  • DNA
  • Hybrid leukemia
  • Ig genes
  • T-cell receptor

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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