Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009

Franco Maria Ruggeri, Roberto Delogu, Tatiana Petouchoff, Olga Tcheremenskaia, Simona De Petris, Lucia Fiore, Maria Grazia Pompa, Francesca Russo, Francesca Zanella, Dario Cesco, Paola Sartore, Monica Micera, Carla Zotti, Elena Cacello, Luciano Balbi, Maria Barbi, Sandro Binda, Valeria Primache, Anna Maria Iorio, Barbara Camilloni & 31 others Michela Basileo, Maria Cristina Medici, Carlo Chezzi, Laura Abelli, Caterina Rizzo, Giovanni Maurizio Giammanco, Simona De Grazia, Maria Angela Platia, Maria Chironna, Anna Sallustio, Cinzia Germinario, Maria Luisa Tanzi, Paola Pietrosemoli, Marcello D’Errico, Anna Marigliano, Nicola Comodo, Chiara Lorini, Vito Martella, Sonia Storelli, Priscilla Cocchi, Filippo Festini, Marta De Rosa, Mattia Chisari, Annibale Raglio, Alessandra Zanchi, Manuela Onori, Gaetano Danzi, Tiziana Lazzarotto, Mino Pedroni, Filippo Ansaldi, The RotaNet-Italy Study Group

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The surveillance network RotaNet-Italia was established in 2007 in order to investigate the diversity of co-circulating rotavirus strains in Italy, and to provide a baseline for future assessment of possible effects of vaccine implementation in selecting novel versus common rotavirus strains. A total of 2,645 rotavirus strains from pediatric patients with acute diarrhea were collected over three consecutive seasons from September 2006 through August 2009, and partially characterized by standardized multiplex RT-PCR. Most of strains (89.1%) belonged to genotypes G1-G4, and G9, associated with either P[8] or P[4], commonly found in humans worldwide. However, in at least 2.0% of cases, viruses exhibited either a G or P type typical of animal viral strains, suggesting gene reassortment events between rotaviruses of different origin. Mixed infections with two or more rotavirus strains were observed frequently (7.6% of patients), and depended on the frequencies of co-circulating rotaviruses of one particular genotype. The numbers and genotypes of likely natural reassortants of common genotype rotaviruses were found to be correlated with the observed numbers and genotypes of mixed infections. Large variation in the relative frequency of different rotavirus genotypes was observed between different seasons and/or areas of Italy, suggesting independent evolution or differential introduction of viral strains with respect to both time and space.

Original languageEnglish
Pages (from-to)1657-1668
Number of pages12
JournalJournal of Medical Virology
Volume83
Issue number9
DOIs
Publication statusPublished - 2011

Fingerprint

Rotavirus
Italy
Diarrhea
Genotype
Coinfection
Multiplex Polymerase Chain Reaction
Vaccines
Pediatrics
Viruses
Genes

Keywords

  • Gene reassortment
  • Genotyping
  • Molecular epidemiology
  • Rotavirus
  • Zoonotic transmission

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Ruggeri, F. M., Delogu, R., Petouchoff, T., Tcheremenskaia, O., De Petris, S., Fiore, L., ... The RotaNet-Italy Study Group (2011). Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009. Journal of Medical Virology, 83(9), 1657-1668. https://doi.org/10.1002/jmv.22163

Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009. / Ruggeri, Franco Maria; Delogu, Roberto; Petouchoff, Tatiana; Tcheremenskaia, Olga; De Petris, Simona; Fiore, Lucia; Pompa, Maria Grazia; Russo, Francesca; Zanella, Francesca; Cesco, Dario; Sartore, Paola; Micera, Monica; Zotti, Carla; Cacello, Elena; Balbi, Luciano; Barbi, Maria; Binda, Sandro; Primache, Valeria; Iorio, Anna Maria; Camilloni, Barbara; Basileo, Michela; Medici, Maria Cristina; Chezzi, Carlo; Abelli, Laura; Rizzo, Caterina; Giammanco, Giovanni Maurizio; De Grazia, Simona; Platia, Maria Angela; Chironna, Maria; Sallustio, Anna; Germinario, Cinzia; Tanzi, Maria Luisa; Pietrosemoli, Paola; D’Errico, Marcello; Marigliano, Anna; Comodo, Nicola; Lorini, Chiara; Martella, Vito; Storelli, Sonia; Cocchi, Priscilla; Festini, Filippo; De Rosa, Marta; Chisari, Mattia; Raglio, Annibale; Zanchi, Alessandra; Onori, Manuela; Danzi, Gaetano; Lazzarotto, Tiziana; Pedroni, Mino; Ansaldi, Filippo; The RotaNet-Italy Study Group.

In: Journal of Medical Virology, Vol. 83, No. 9, 2011, p. 1657-1668.

Research output: Contribution to journalArticle

Ruggeri, FM, Delogu, R, Petouchoff, T, Tcheremenskaia, O, De Petris, S, Fiore, L, Pompa, MG, Russo, F, Zanella, F, Cesco, D, Sartore, P, Micera, M, Zotti, C, Cacello, E, Balbi, L, Barbi, M, Binda, S, Primache, V, Iorio, AM, Camilloni, B, Basileo, M, Medici, MC, Chezzi, C, Abelli, L, Rizzo, C, Giammanco, GM, De Grazia, S, Platia, MA, Chironna, M, Sallustio, A, Germinario, C, Tanzi, ML, Pietrosemoli, P, D’Errico, M, Marigliano, A, Comodo, N, Lorini, C, Martella, V, Storelli, S, Cocchi, P, Festini, F, De Rosa, M, Chisari, M, Raglio, A, Zanchi, A, Onori, M, Danzi, G, Lazzarotto, T, Pedroni, M, Ansaldi, F & The RotaNet-Italy Study Group 2011, 'Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009', Journal of Medical Virology, vol. 83, no. 9, pp. 1657-1668. https://doi.org/10.1002/jmv.22163
Ruggeri FM, Delogu R, Petouchoff T, Tcheremenskaia O, De Petris S, Fiore L et al. Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009. Journal of Medical Virology. 2011;83(9):1657-1668. https://doi.org/10.1002/jmv.22163
Ruggeri, Franco Maria ; Delogu, Roberto ; Petouchoff, Tatiana ; Tcheremenskaia, Olga ; De Petris, Simona ; Fiore, Lucia ; Pompa, Maria Grazia ; Russo, Francesca ; Zanella, Francesca ; Cesco, Dario ; Sartore, Paola ; Micera, Monica ; Zotti, Carla ; Cacello, Elena ; Balbi, Luciano ; Barbi, Maria ; Binda, Sandro ; Primache, Valeria ; Iorio, Anna Maria ; Camilloni, Barbara ; Basileo, Michela ; Medici, Maria Cristina ; Chezzi, Carlo ; Abelli, Laura ; Rizzo, Caterina ; Giammanco, Giovanni Maurizio ; De Grazia, Simona ; Platia, Maria Angela ; Chironna, Maria ; Sallustio, Anna ; Germinario, Cinzia ; Tanzi, Maria Luisa ; Pietrosemoli, Paola ; D’Errico, Marcello ; Marigliano, Anna ; Comodo, Nicola ; Lorini, Chiara ; Martella, Vito ; Storelli, Sonia ; Cocchi, Priscilla ; Festini, Filippo ; De Rosa, Marta ; Chisari, Mattia ; Raglio, Annibale ; Zanchi, Alessandra ; Onori, Manuela ; Danzi, Gaetano ; Lazzarotto, Tiziana ; Pedroni, Mino ; Ansaldi, Filippo ; The RotaNet-Italy Study Group. / Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009. In: Journal of Medical Virology. 2011 ; Vol. 83, No. 9. pp. 1657-1668.
@article{00c17d275ebe4c4ebdaa2395451c5ecf,
title = "Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009",
abstract = "The surveillance network RotaNet-Italia was established in 2007 in order to investigate the diversity of co-circulating rotavirus strains in Italy, and to provide a baseline for future assessment of possible effects of vaccine implementation in selecting novel versus common rotavirus strains. A total of 2,645 rotavirus strains from pediatric patients with acute diarrhea were collected over three consecutive seasons from September 2006 through August 2009, and partially characterized by standardized multiplex RT-PCR. Most of strains (89.1{\%}) belonged to genotypes G1-G4, and G9, associated with either P[8] or P[4], commonly found in humans worldwide. However, in at least 2.0{\%} of cases, viruses exhibited either a G or P type typical of animal viral strains, suggesting gene reassortment events between rotaviruses of different origin. Mixed infections with two or more rotavirus strains were observed frequently (7.6{\%} of patients), and depended on the frequencies of co-circulating rotaviruses of one particular genotype. The numbers and genotypes of likely natural reassortants of common genotype rotaviruses were found to be correlated with the observed numbers and genotypes of mixed infections. Large variation in the relative frequency of different rotavirus genotypes was observed between different seasons and/or areas of Italy, suggesting independent evolution or differential introduction of viral strains with respect to both time and space.",
keywords = "Gene reassortment, Genotyping, Molecular epidemiology, Rotavirus, Zoonotic transmission",
author = "Ruggeri, {Franco Maria} and Roberto Delogu and Tatiana Petouchoff and Olga Tcheremenskaia and {De Petris}, Simona and Lucia Fiore and Pompa, {Maria Grazia} and Francesca Russo and Francesca Zanella and Dario Cesco and Paola Sartore and Monica Micera and Carla Zotti and Elena Cacello and Luciano Balbi and Maria Barbi and Sandro Binda and Valeria Primache and Iorio, {Anna Maria} and Barbara Camilloni and Michela Basileo and Medici, {Maria Cristina} and Carlo Chezzi and Laura Abelli and Caterina Rizzo and Giammanco, {Giovanni Maurizio} and {De Grazia}, Simona and Platia, {Maria Angela} and Maria Chironna and Anna Sallustio and Cinzia Germinario and Tanzi, {Maria Luisa} and Paola Pietrosemoli and Marcello D’Errico and Anna Marigliano and Nicola Comodo and Chiara Lorini and Vito Martella and Sonia Storelli and Priscilla Cocchi and Filippo Festini and {De Rosa}, Marta and Mattia Chisari and Annibale Raglio and Alessandra Zanchi and Manuela Onori and Gaetano Danzi and Tiziana Lazzarotto and Mino Pedroni and Filippo Ansaldi and {The RotaNet-Italy Study Group}",
year = "2011",
doi = "10.1002/jmv.22163",
language = "English",
volume = "83",
pages = "1657--1668",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "9",

}

TY - JOUR

T1 - Molecular characterization of rotavirus strains from children with Diarrhea in Italy 2007-2009

AU - Ruggeri, Franco Maria

AU - Delogu, Roberto

AU - Petouchoff, Tatiana

AU - Tcheremenskaia, Olga

AU - De Petris, Simona

AU - Fiore, Lucia

AU - Pompa, Maria Grazia

AU - Russo, Francesca

AU - Zanella, Francesca

AU - Cesco, Dario

AU - Sartore, Paola

AU - Micera, Monica

AU - Zotti, Carla

AU - Cacello, Elena

AU - Balbi, Luciano

AU - Barbi, Maria

AU - Binda, Sandro

AU - Primache, Valeria

AU - Iorio, Anna Maria

AU - Camilloni, Barbara

AU - Basileo, Michela

AU - Medici, Maria Cristina

AU - Chezzi, Carlo

AU - Abelli, Laura

AU - Rizzo, Caterina

AU - Giammanco, Giovanni Maurizio

AU - De Grazia, Simona

AU - Platia, Maria Angela

AU - Chironna, Maria

AU - Sallustio, Anna

AU - Germinario, Cinzia

AU - Tanzi, Maria Luisa

AU - Pietrosemoli, Paola

AU - D’Errico, Marcello

AU - Marigliano, Anna

AU - Comodo, Nicola

AU - Lorini, Chiara

AU - Martella, Vito

AU - Storelli, Sonia

AU - Cocchi, Priscilla

AU - Festini, Filippo

AU - De Rosa, Marta

AU - Chisari, Mattia

AU - Raglio, Annibale

AU - Zanchi, Alessandra

AU - Onori, Manuela

AU - Danzi, Gaetano

AU - Lazzarotto, Tiziana

AU - Pedroni, Mino

AU - Ansaldi, Filippo

AU - The RotaNet-Italy Study Group

PY - 2011

Y1 - 2011

N2 - The surveillance network RotaNet-Italia was established in 2007 in order to investigate the diversity of co-circulating rotavirus strains in Italy, and to provide a baseline for future assessment of possible effects of vaccine implementation in selecting novel versus common rotavirus strains. A total of 2,645 rotavirus strains from pediatric patients with acute diarrhea were collected over three consecutive seasons from September 2006 through August 2009, and partially characterized by standardized multiplex RT-PCR. Most of strains (89.1%) belonged to genotypes G1-G4, and G9, associated with either P[8] or P[4], commonly found in humans worldwide. However, in at least 2.0% of cases, viruses exhibited either a G or P type typical of animal viral strains, suggesting gene reassortment events between rotaviruses of different origin. Mixed infections with two or more rotavirus strains were observed frequently (7.6% of patients), and depended on the frequencies of co-circulating rotaviruses of one particular genotype. The numbers and genotypes of likely natural reassortants of common genotype rotaviruses were found to be correlated with the observed numbers and genotypes of mixed infections. Large variation in the relative frequency of different rotavirus genotypes was observed between different seasons and/or areas of Italy, suggesting independent evolution or differential introduction of viral strains with respect to both time and space.

AB - The surveillance network RotaNet-Italia was established in 2007 in order to investigate the diversity of co-circulating rotavirus strains in Italy, and to provide a baseline for future assessment of possible effects of vaccine implementation in selecting novel versus common rotavirus strains. A total of 2,645 rotavirus strains from pediatric patients with acute diarrhea were collected over three consecutive seasons from September 2006 through August 2009, and partially characterized by standardized multiplex RT-PCR. Most of strains (89.1%) belonged to genotypes G1-G4, and G9, associated with either P[8] or P[4], commonly found in humans worldwide. However, in at least 2.0% of cases, viruses exhibited either a G or P type typical of animal viral strains, suggesting gene reassortment events between rotaviruses of different origin. Mixed infections with two or more rotavirus strains were observed frequently (7.6% of patients), and depended on the frequencies of co-circulating rotaviruses of one particular genotype. The numbers and genotypes of likely natural reassortants of common genotype rotaviruses were found to be correlated with the observed numbers and genotypes of mixed infections. Large variation in the relative frequency of different rotavirus genotypes was observed between different seasons and/or areas of Italy, suggesting independent evolution or differential introduction of viral strains with respect to both time and space.

KW - Gene reassortment

KW - Genotyping

KW - Molecular epidemiology

KW - Rotavirus

KW - Zoonotic transmission

UR - http://www.scopus.com/inward/record.url?scp=79960146343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960146343&partnerID=8YFLogxK

U2 - 10.1002/jmv.22163

DO - 10.1002/jmv.22163

M3 - Article

VL - 83

SP - 1657

EP - 1668

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 9

ER -