Molecular characterization of virulence determinants of Stenotrophomonas maltophilia strains isolated from patients affected by cystic fibrosis

G. Di Bonaventura, G. Prosseda, F. Del Chierico, S. Cannavacciuolo, P. Cipriani, A. Petrucca, F. Superti, M. G. Ammendolia, C. Concato, E. Fiscarelli, M. Casalino, R. Piccolomini, M. Nicoletti, Bianca Colonna

Research output: Contribution to journalArticle

Abstract

Stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen which is currently isolated with increasing frequency from the airways of cystic fibrosis (CF) patients. In this study 13 S. maltophilia strains (11 isolated from the airways of independent CF patients, and two non-CF respiratory reference strains) have been characterized for the expression of several virulence-associated factors. In particular, the ability to form biofilm on abiotic surfaces has been determined and correlated with different features, such as motility, adherence and the ability to invade A549 respiratory epithelial cells. Moreover, the presence of a flagellum-associated gene as well as that of the StmPr1 gene, which encodes an extra-cellular protease, have been determined by Southern blot hybridization. Our data indicate that the different degree of biofilm formation exhibited by the 11 CF isolates does not correlate with motility, ability to adhere to and invade A549 cells, or with the presence of flagella. On the other hand, among the CF isolates the StmPr1 gene was found only in two strains, both able to establish chronic lung infections in CF patients. Moreover, only four of the strains analyzed show a temperature-independent antibiotic-resistance profile, suggesting either a different origin of these strains or an intervening adaptation to host tissues.

Original languageEnglish
Pages (from-to)529-537
Number of pages9
JournalInternational Journal of Immunopathology and Pharmacology
Volume20
Issue number3
Publication statusPublished - Jul 2007

Fingerprint

Stenotrophomonas maltophilia
Cystic Fibrosis
Virulence
Flagella
Biofilms
Genes
Virulence Factors
Microbial Drug Resistance
Southern Blotting
Fibrosis
Peptide Hydrolases
Epithelial Cells
Lung
Temperature
Infection

Keywords

  • Adherence
  • Antibiotic resistance
  • Biofilm
  • Cystic fibrosis
  • Invasivity
  • Stenotrophomonas maltophilia
  • Virulence factors

ASJC Scopus subject areas

  • Pharmacology

Cite this

Molecular characterization of virulence determinants of Stenotrophomonas maltophilia strains isolated from patients affected by cystic fibrosis. / Di Bonaventura, G.; Prosseda, G.; Del Chierico, F.; Cannavacciuolo, S.; Cipriani, P.; Petrucca, A.; Superti, F.; Ammendolia, M. G.; Concato, C.; Fiscarelli, E.; Casalino, M.; Piccolomini, R.; Nicoletti, M.; Colonna, Bianca.

In: International Journal of Immunopathology and Pharmacology, Vol. 20, No. 3, 07.2007, p. 529-537.

Research output: Contribution to journalArticle

Di Bonaventura, G, Prosseda, G, Del Chierico, F, Cannavacciuolo, S, Cipriani, P, Petrucca, A, Superti, F, Ammendolia, MG, Concato, C, Fiscarelli, E, Casalino, M, Piccolomini, R, Nicoletti, M & Colonna, B 2007, 'Molecular characterization of virulence determinants of Stenotrophomonas maltophilia strains isolated from patients affected by cystic fibrosis', International Journal of Immunopathology and Pharmacology, vol. 20, no. 3, pp. 529-537.
Di Bonaventura, G. ; Prosseda, G. ; Del Chierico, F. ; Cannavacciuolo, S. ; Cipriani, P. ; Petrucca, A. ; Superti, F. ; Ammendolia, M. G. ; Concato, C. ; Fiscarelli, E. ; Casalino, M. ; Piccolomini, R. ; Nicoletti, M. ; Colonna, Bianca. / Molecular characterization of virulence determinants of Stenotrophomonas maltophilia strains isolated from patients affected by cystic fibrosis. In: International Journal of Immunopathology and Pharmacology. 2007 ; Vol. 20, No. 3. pp. 529-537.
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