Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma

Francesco Carinci, Diego Arcelli, Lorenzo Lo Muzio, Francesca Francioso, Davide Valentini, Rita Evangelisti, Stefano Volinia, Anna D'Angelo, Germana Meroni, Massimo Zollo, Antonio Pastore, Franco Ionna, Filiberto Mastrangelo, Pio Conti, Stefano Tetè

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Classification and prognosis of larynx squamous cell carcinoma (LSCC) depends on clinical and histopathological examination. Currently, expression profiling harbors the potential to investigate, classify, and better manage cancer. Gene expression profiles of 22 primary LSCCs were analyzed by microarrays containing 19,200 cDNAs. GOAL functionally classified differentially expressed genes, and a novel "in silico" procedure identified physical gene clusters differentially transcribed. A signature of 158 genes differentiated tumors with nodal metastasis. A novel statistical method allowed categorization of metastatic tumors into 2 distinct subgroups of differential gene expression patterns. Among genes correlated to nodal metastatic progression, we verified in vitro that NM23-H3 reduced cell motility and TRIM8 were a growth suppressor. Six chromosomal regions were specifically downregulated in metastatic tumors. This large-scale gene expression analysis in LSCC provides information on changes in genomic activity associated with lymphonodal metastasis and identifies molecules that might prove useful as novel therapeutic targets.

Original languageEnglish
Pages (from-to)233-245
Number of pages13
JournalTranslational Research
Volume150
Issue number4
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Larynx
Squamous Cell Carcinoma
Genes
Gene expression
Neoplasm Metastasis
Tumors
Neoplasms
Gene Expression
Microarrays
Multigene Family
Ports and harbors
Transcriptome
Computer Simulation
Cell Movement
Statistical methods
Down-Regulation
Complementary DNA
Molecules
Epithelial Cells
Growth

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, medical
  • Public Health, Environmental and Occupational Health

Cite this

Carinci, F., Arcelli, D., Lo Muzio, L., Francioso, F., Valentini, D., Evangelisti, R., ... Tetè, S. (2007). Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma. Translational Research, 150(4), 233-245. https://doi.org/10.1016/j.trsl.2007.03.011

Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma. / Carinci, Francesco; Arcelli, Diego; Lo Muzio, Lorenzo; Francioso, Francesca; Valentini, Davide; Evangelisti, Rita; Volinia, Stefano; D'Angelo, Anna; Meroni, Germana; Zollo, Massimo; Pastore, Antonio; Ionna, Franco; Mastrangelo, Filiberto; Conti, Pio; Tetè, Stefano.

In: Translational Research, Vol. 150, No. 4, 10.2007, p. 233-245.

Research output: Contribution to journalArticle

Carinci, F, Arcelli, D, Lo Muzio, L, Francioso, F, Valentini, D, Evangelisti, R, Volinia, S, D'Angelo, A, Meroni, G, Zollo, M, Pastore, A, Ionna, F, Mastrangelo, F, Conti, P & Tetè, S 2007, 'Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma', Translational Research, vol. 150, no. 4, pp. 233-245. https://doi.org/10.1016/j.trsl.2007.03.011
Carinci F, Arcelli D, Lo Muzio L, Francioso F, Valentini D, Evangelisti R et al. Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma. Translational Research. 2007 Oct;150(4):233-245. https://doi.org/10.1016/j.trsl.2007.03.011
Carinci, Francesco ; Arcelli, Diego ; Lo Muzio, Lorenzo ; Francioso, Francesca ; Valentini, Davide ; Evangelisti, Rita ; Volinia, Stefano ; D'Angelo, Anna ; Meroni, Germana ; Zollo, Massimo ; Pastore, Antonio ; Ionna, Franco ; Mastrangelo, Filiberto ; Conti, Pio ; Tetè, Stefano. / Molecular classification of nodal metastasis in primary larynx squamous cell carcinoma. In: Translational Research. 2007 ; Vol. 150, No. 4. pp. 233-245.
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