Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family

E. Monti, M. T. Bassi, R. Bresciani, S. Civini, G. L. Croci, N. Papini, M. Riboni, G. Zanchetti, A. Ballabio, A. Preti, G. Tettamanti, B. Venerando, G. Borsani

Research output: Contribution to journalArticle

Abstract

Several mammalian sialidases have been cloned so far and here we describe the identification and expression of a new member of the human sialidase gene family. The NEU4 gene, identified by searching sequence databases for entries showing homologies to the human cytosolic sialidase NEU2, maps in 2q37 and encodes a 484-residue protein. The polypeptide contains all the typical sialidase amino acid motifs and, apart from an amino acid stretch that appears unique among mammalian sialidases, shows a high degree of homology for NEU2 and the plasma membrane-associated (NEU3) sialidases. RNA dot-blot analysis showed a low but wide expression pattern, with the highest level in liver. Transient transfection in COS7 cells allowed the detection of a sialidase activity toward the artificial substrate 4MU-NeuAc in the acidic range of pH. Immunofluorescence staining and Western blot analysis demonstrated the association of NEU4 with the inner cell membranes.

Original languageEnglish
Pages (from-to)445-453
Number of pages9
JournalGenomics
Volume83
Issue number3
DOIs
Publication statusPublished - Mar 2004

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Keywords

  • Gene expression profile
  • Gene structure
  • Human sialidase
  • Subcellular localization
  • Transient transfection

ASJC Scopus subject areas

  • Genetics

Cite this

Monti, E., Bassi, M. T., Bresciani, R., Civini, S., Croci, G. L., Papini, N., Riboni, M., Zanchetti, G., Ballabio, A., Preti, A., Tettamanti, G., Venerando, B., & Borsani, G. (2004). Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family. Genomics, 83(3), 445-453. https://doi.org/10.1016/j.ygeno.2003.08.019