Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family

Furio Spano, Lorenza Putignani, Silvia Naitza, Claudia Puri, Steve Wright, Andrea Crisanti

Research output: Contribution to journalArticle

Abstract

The apicomplexan parasite Cryptosporidium parvum invades and multiplies primarily in the brush border cells of the intestinal mucosa causing in AIDS patients a severe diarrhoea that represents a significant contributing factor leading to death. Morphological analysis indicates that the invasion machinery of C. parvum is similar to the apical complex of other parasites of the phylum Apicomplexa. We provide here evidence indicating that C. parvum also shares with these parasites a molecule crucial for the invasion of host cells. We have cloned a 3894 bp-long C. parvum cDNA encoding a protein characterised by sequence and structural similarities with members of the thrombospondin (TSP) family previously described in apicomplexan parasites of the genera Toxoplasma, Eimeria and Plasmodium. This novel C. parvum molecule, the TSP-related adhesive protein of Cryptosporidium-1 (TRAP-C1), is encoded by a single copy gene containing no introns. TRAP-C1 is localised in the apical end of C. parvum sporozoites and is structurally related to the micronemal proteins MIC2 of Toxoplasma and Etp100 of Eimeria, which are involved in host-cell attachment and/or invasion. The identification of TRAP-C1 sheds new light on the molecules possibly involved in the invasion process of intestinal cells by C. parvum. We have also analysed the sequence variation of TRAP-C1 among C. parvum isolates and in the closely related species C. wrairi. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)147-162
Number of pages16
JournalMolecular and Biochemical Parasitology
Volume92
Issue number1
DOIs
Publication statusPublished - Apr 1 1998

Fingerprint

Thrombospondins
Cryptosporidium parvum
Molecular Cloning
Cryptosporidium
Adhesives
Genes
Parasites
Eimeria
Toxoplasma
Proteins
Apicomplexa
Sporozoites
Plasmodium
Intestinal Mucosa
Microvilli
Introns
Diarrhea
Acquired Immunodeficiency Syndrome
Complementary DNA

Keywords

  • Apical complex
  • cDNA
  • Cryptosporidium parvum
  • Cryptosporidium wrairi
  • Sporozoite
  • Sulfatide binding domain
  • TRAP-C1

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family. / Spano, Furio; Putignani, Lorenza; Naitza, Silvia; Puri, Claudia; Wright, Steve; Crisanti, Andrea.

In: Molecular and Biochemical Parasitology, Vol. 92, No. 1, 01.04.1998, p. 147-162.

Research output: Contribution to journalArticle

Spano, Furio ; Putignani, Lorenza ; Naitza, Silvia ; Puri, Claudia ; Wright, Steve ; Crisanti, Andrea. / Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family. In: Molecular and Biochemical Parasitology. 1998 ; Vol. 92, No. 1. pp. 147-162.
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