Molecular cloning and identification of murine caspase-8

Marc Van De Craen, Geert Van Loo, Wim Declercq, Peter Schotte, Ilse Van Den Brande, Susanna Mandruzzato, Pierre Van Der Bruggen, Walter Fiers, Peter Vandenabeele

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Several caspases are mediators of apoptotic cell death. We describe a novel murine member of this growing protein family. Based on homology and especially on the substrate specificity, this new procaspase is identified as the murine counterpart of human procaspase-8. The protein exhibits a rather low similarity (76%) and identity (70%) to human procaspase-8. Procaspase-8 mRNA is expressed in all adult mouse tissues examined, the highest levels being reached in kidney, liver and lung. Procaspase-8 mRNA expression is highest in seven-day old embryos, but also during later stages of development the expression was fairly high. Both human and murine procaspase-8 are very weak substrates for granzyme B as compared to procaspase-3. Murine procaspases-1, 2, 3, 6, 7, 8, 11/4 and 12 are processed by recombinant murine caspase-8, suggesting a key role in the procaspase activation cascade. In addition, murine caspase-8 induced cell death that was inhibited both by cytokine response modifier A and p35. In vitro experiments demonstrated that p35 inhibits caspase-8 directly.

Original languageEnglish
Pages (from-to)1017-1026
Number of pages10
JournalJournal of Molecular Biology
Volume284
Issue number4
DOIs
Publication statusPublished - Dec 11 1998

Fingerprint

Caspase 8
Molecular Cloning
Cell Death
Caspase 2
Granzymes
Caspase 1
Messenger RNA
Caspases
Substrate Specificity
Caspase 3
Proteins
Embryonic Structures
Cytokines
Kidney
Lung
Liver

Keywords

  • Apoptosis
  • Caspase-8
  • FLICE
  • Granzyme B
  • p35

ASJC Scopus subject areas

  • Virology

Cite this

Van De Craen, M., Van Loo, G., Declercq, W., Schotte, P., Van Den Brande, I., Mandruzzato, S., ... Vandenabeele, P. (1998). Molecular cloning and identification of murine caspase-8. Journal of Molecular Biology, 284(4), 1017-1026. https://doi.org/10.1006/jmbi.1998.2226

Molecular cloning and identification of murine caspase-8. / Van De Craen, Marc; Van Loo, Geert; Declercq, Wim; Schotte, Peter; Van Den Brande, Ilse; Mandruzzato, Susanna; Van Der Bruggen, Pierre; Fiers, Walter; Vandenabeele, Peter.

In: Journal of Molecular Biology, Vol. 284, No. 4, 11.12.1998, p. 1017-1026.

Research output: Contribution to journalArticle

Van De Craen, M, Van Loo, G, Declercq, W, Schotte, P, Van Den Brande, I, Mandruzzato, S, Van Der Bruggen, P, Fiers, W & Vandenabeele, P 1998, 'Molecular cloning and identification of murine caspase-8', Journal of Molecular Biology, vol. 284, no. 4, pp. 1017-1026. https://doi.org/10.1006/jmbi.1998.2226
Van De Craen M, Van Loo G, Declercq W, Schotte P, Van Den Brande I, Mandruzzato S et al. Molecular cloning and identification of murine caspase-8. Journal of Molecular Biology. 1998 Dec 11;284(4):1017-1026. https://doi.org/10.1006/jmbi.1998.2226
Van De Craen, Marc ; Van Loo, Geert ; Declercq, Wim ; Schotte, Peter ; Van Den Brande, Ilse ; Mandruzzato, Susanna ; Van Der Bruggen, Pierre ; Fiers, Walter ; Vandenabeele, Peter. / Molecular cloning and identification of murine caspase-8. In: Journal of Molecular Biology. 1998 ; Vol. 284, No. 4. pp. 1017-1026.
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