Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase.

Y. Matsubara, J. P. Kraus, H. Ozasa, R. Glassberg, G. Finocchiaro, Y. Ikeda, J. Mole, L. E. Rosenberg, K. Tanaka

Research output: Contribution to journalArticle

Abstract

cDNA encoding the precursor of rat liver medium chain acyl-CoA dehydrogenase (EC 1.3.99.3) was cloned and sequenced. The longest cDNA insert isolated was 1866 bases in length. This cDNA encodes the entire protein of 421-amino acids including a 25-amino acid leader peptide and a 396-amino acid mature polypeptide. The identity of the medium chain acyl-CoA dehydrogenase clone was confirmed by matching the amino acid sequence predicted from the cDNA to the NH2-terminal and nine internal tryptic peptide sequences derived from pure rat liver medium chain acyl-CoA dehydrogenase. The calculated molecular masses of the precursor medium chain acyl-CoA dehydrogenase, the mature medium chain acyl-CoA dehydrogenase, and the leader peptide are 46,600, 43,700, and 2,900 daltons, respectively. The leader peptide contains five basic amino acids and only one acidic amino acid; thus, it is positively charged, overall. Cysteine residues are unevenly distributed in the mature portion of the protein; five of six are found within the NH2-terminal half of the polypeptide. Comparison of medium chain acyl-CoA dehydrogenase sequence to other flavoproteins and enzymes which act on coenzyme A ester substrates did not lead to unambiguous identification of a possible FAD-binding site nor a coenzyme A-binding domain. The sequencing of other homologous acyl-CoA dehydrogenases will be informative in this regard.

Original languageEnglish
Pages (from-to)10104-10108
Number of pages5
JournalJournal of Biological Chemistry
Volume262
Issue number21
Publication statusPublished - Jul 25 1987

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Acyl-CoA Dehydrogenase
Cloning
Molecular Cloning
Liver
Rats
Nucleotides
Complementary DNA
Protein Sorting Signals
Amino Acids
Coenzyme A
Peptides
Acyl-CoA Dehydrogenases
Acidic Amino Acids
Flavoproteins
Basic Amino Acids
Mass Media
Flavin-Adenine Dinucleotide
Molecular mass
Cysteine
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase. / Matsubara, Y.; Kraus, J. P.; Ozasa, H.; Glassberg, R.; Finocchiaro, G.; Ikeda, Y.; Mole, J.; Rosenberg, L. E.; Tanaka, K.

In: Journal of Biological Chemistry, Vol. 262, No. 21, 25.07.1987, p. 10104-10108.

Research output: Contribution to journalArticle

Matsubara, Y, Kraus, JP, Ozasa, H, Glassberg, R, Finocchiaro, G, Ikeda, Y, Mole, J, Rosenberg, LE & Tanaka, K 1987, 'Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase.', Journal of Biological Chemistry, vol. 262, no. 21, pp. 10104-10108.
Matsubara, Y. ; Kraus, J. P. ; Ozasa, H. ; Glassberg, R. ; Finocchiaro, G. ; Ikeda, Y. ; Mole, J. ; Rosenberg, L. E. ; Tanaka, K. / Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 21. pp. 10104-10108.
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