Molecular cloning and nucleotide sequence of chicken avidin-related genes 1-5

R. A. Keinanen, M. J. Wallen, P. A. Kristo, M. O. Laukkanen, T. A. Toimela, M. A. Helenius, M. S. Kulomaa

Research output: Contribution to journalArticle

Abstract

Using avidin cDNA as a hybridisation probe, we detected a gene family whose putative products are related to the chicken egg-white avidin. Two overlapping genomic clones were found to contain five genes (avidin-related genes 1-5, avr1-avr5), which have been cloned, characterized and sequenced. All of the genes have a four-exon structure with an overall identity with the avidin cDNA of 88-92%. The genes appear to have no pseudogenic features and, in fact, two of these genes have been shown to be transcribed. The putative proteins share a sequence identity of 68-78% with avidin. The amino acid residues responsible for the biotin-binding activity of avidin and the bacterial biotin-binding protein, streptavidin, are highly conserved. Since avidin is induced in both a progesterone-specific manner and in connection with inflammation, these genes offer a valuable tool to study complex gene regulation in vivo.

Original languageEnglish
Pages (from-to)615-621
Number of pages7
JournalEuropean Journal of Biochemistry
Volume220
Issue number2
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Biochemistry

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    Keinanen, R. A., Wallen, M. J., Kristo, P. A., Laukkanen, M. O., Toimela, T. A., Helenius, M. A., & Kulomaa, M. S. (1994). Molecular cloning and nucleotide sequence of chicken avidin-related genes 1-5. European Journal of Biochemistry, 220(2), 615-621.