Molecular cloning and sequence analysis of a cDNA coding for the mouse alpha-like embryonic globin chain x

Maria Giulia Farace, Alison Hill, Marco Tripodi, Richard W. Padgett, Giuseppe Raschella, Roberto Gambari, Antonio Fantoni, Clyde A. Hutchison, Marshall H. Edgell

Research output: Contribution to journalArticlepeer-review

Abstract

Cytoplasmic poly(A)+mRNA from 12-day mouse-yolk-sac erythroid cells has been used to prepare a cDNA library in the plasmid pBR322. One clone containing sequences coding for the α-like embryonic globin chain x, pHE52, has been identified by hybrid selection and in vitro translation of the complementary mRNA. The nucleotide sequence of pHE52 confirms that it codes for an embryonic α-like globin chain. The insert sequence is 316 nucleotides long, contains the codons corresponding to amino acid residues 43-141, and extends into the 3' untranslated region. An analysis of the nucleotide sequence of pHE52 and the other known α globins suggests that the adult-embryonic divergence began approx. 400 million years ago reflecting a difference in the evolutionary history of the α- and β-globin gene complexes.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalGene
Volume31
Issue number1-3
DOIs
Publication statusPublished - 1984

Keywords

  • evolution
  • gene complexes
  • in vitro translation
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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