Molecular cloning of a recombinant retrovirus carrying a mutated envAKR-mycMH2 fusion gene immortalizing cells of the monocytic-macrophage lineage

M. Sassano, F. Granucci, M. Seveso, G. Marconi, M. Foti, P. Ricciardi-Castagnoli

Research output: Contribution to journalArticle

Abstract

The VN-11 recombinant retroviruses, originally generated by co-transfection of the avian MH2 and AKRv viral genomes, were molecularly cloned from an infected mouse cell line named Nil. The analysis of the proviral genome sequence from one of these recombinants showed a possible envAKR-mycMH2 fusion. Point mutations were also found in this envAKR-mycMH2 gene. The cloned viral genome was co-transfected with the neo gene into the ψ2 packaging cell line. Selected clones were shown to transcribe the viral genome and supernatants from these cultures, containing C-type particles, were used to infect primary cultures from mouse lymphoid tissues and brain. Proliferating macrophages and microglial cell clones were obtained, indicating that various types of cells of the mouse monocytic-macrophage lineage can be immortalized in spite of the absence of selection or special growth conditions.

Original languageEnglish
Pages (from-to)1473-1477
Number of pages5
JournalOncogene
Volume9
Issue number5
Publication statusPublished - May 1994

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Viral Genome
Gene Fusion
Molecular Cloning
Retroviridae
Macrophages
Clone Cells
Cell Line
Lymphoid Tissue
Product Packaging
Point Mutation
Genes
Transfection
Genome
Brain
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Sassano, M., Granucci, F., Seveso, M., Marconi, G., Foti, M., & Ricciardi-Castagnoli, P. (1994). Molecular cloning of a recombinant retrovirus carrying a mutated envAKR-mycMH2 fusion gene immortalizing cells of the monocytic-macrophage lineage. Oncogene, 9(5), 1473-1477.

Molecular cloning of a recombinant retrovirus carrying a mutated envAKR-mycMH2 fusion gene immortalizing cells of the monocytic-macrophage lineage. / Sassano, M.; Granucci, F.; Seveso, M.; Marconi, G.; Foti, M.; Ricciardi-Castagnoli, P.

In: Oncogene, Vol. 9, No. 5, 05.1994, p. 1473-1477.

Research output: Contribution to journalArticle

Sassano, M, Granucci, F, Seveso, M, Marconi, G, Foti, M & Ricciardi-Castagnoli, P 1994, 'Molecular cloning of a recombinant retrovirus carrying a mutated envAKR-mycMH2 fusion gene immortalizing cells of the monocytic-macrophage lineage', Oncogene, vol. 9, no. 5, pp. 1473-1477.
Sassano M, Granucci F, Seveso M, Marconi G, Foti M, Ricciardi-Castagnoli P. Molecular cloning of a recombinant retrovirus carrying a mutated envAKR-mycMH2 fusion gene immortalizing cells of the monocytic-macrophage lineage. Oncogene. 1994 May;9(5):1473-1477.
Sassano, M. ; Granucci, F. ; Seveso, M. ; Marconi, G. ; Foti, M. ; Ricciardi-Castagnoli, P. / Molecular cloning of a recombinant retrovirus carrying a mutated envAKR-mycMH2 fusion gene immortalizing cells of the monocytic-macrophage lineage. In: Oncogene. 1994 ; Vol. 9, No. 5. pp. 1473-1477.
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