Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family

B. W M Richter, S. S. Mir, L. J. Eiben, J. Lewis, S. B. Reffey, A. Frattini, L. Tian, S. Frank, R. J. Youle, D. L. Nelson, L. D. Notarangelo, P. Vezzoni, H. O. Fearnhead, C. S. Duckett

Research output: Contribution to journalArticle

Abstract

Inhibitor of apoptosis protein (IAP)-like protein-1 (ILP-1) (also known as X-linked IAP [XIAP] and mammalian IAP homolog A [MIHA]) is a potent inhibitor of apoptosis and exerts its effects, at least in part, by the direct association with and inhibition of specific caspases. Here, we describe the molecular cloning and characterization of a human gene related to ILP-1, termed ILP-2. Despite high homology to ILP-1, ILP-2 is encoded by a distinct gene, which in normal tissues is expressed solely in testis. In contrast to ILP-1, overexpression of ILP-2 had no protective effect on apoptosis mediated by Fas (also known as CD95) or tumor necrosis factor; However, ILP-2 potently inhibited apoptosis induced by overexpression of Bax or by coexpression of caspase 9 with Apaf-1, and preincubation of cytosolic extracts with ILP-2 abrogated caspase activation in vitro. A processed form of caspase 9 could be coprecipitated with ILP-2 from cells, suggesting a physical interaction between ILP-2 and caspase 9. Thus, ILP-2 is a novel IAP family member with restricted specificity for caspase 9.

Original languageEnglish
Pages (from-to)4292-4301
Number of pages10
JournalMolecular and Cellular Biology
Volume21
Issue number13
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family'. Together they form a unique fingerprint.

  • Cite this

    Richter, B. W. M., Mir, S. S., Eiben, L. J., Lewis, J., Reffey, S. B., Frattini, A., Tian, L., Frank, S., Youle, R. J., Nelson, D. L., Notarangelo, L. D., Vezzoni, P., Fearnhead, H. O., & Duckett, C. S. (2001). Molecular cloning of ILP-2, a novel member of the inhibitor of apoptosis protein family. Molecular and Cellular Biology, 21(13), 4292-4301. https://doi.org/10.1128/MCB.21.13.4292-4301.2001