Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues

Monica Napolitano, Alessandra Zingoni, Giovanni Bernardini, Gaia Spinetti, Anna Nista, Clelia T. Storlazzi, Mariano Rocchi, Angela Santoni

Research output: Contribution to journalArticle

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Abstract

Several chemokine receptors have been cloned and shown to belong to a superfamily of seven transmembrane, G protein-coupled receptors. We report here the molecular cloning of TER1, a novel human chemokine receptor-like gene. The amino acid sequence deduced from the TER1 cDNA shows 43, 40, 40, and 39% identity to CCR4, CCR5, CCR1, and CCR2B β chemokine receptors, respectively. By the use of fluorescent in situ hybridization, we have mapped the TER1 gene to chromosome 3p21, clustered with other chemokine receptor genes. By Northern blot analysis, TER1 mRNA is found to be expressed in the thymus, spleen, and at barely detectable levels in peripheral blood lymphocytes. Moreover, TER1 message is abundant in the NK cell line NK3.3 and in the T cell line MOLT-4. The restricted TER1 expression in cells and tissues of the lymphoid lineage suggests that this receptor may play a role in regulating immune functions.

Original languageEnglish
Pages (from-to)2759-2763
Number of pages5
JournalJournal of Immunology
Volume157
Issue number7
Publication statusPublished - Oct 1 1996

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Chemokine Receptors
Molecular Cloning
Lymphoid Tissue
Genes
Lymphocytes
Cell Line
Fluorescence In Situ Hybridization
Natural Killer Cells
Northern Blotting
Thymus Gland
Amino Acid Sequence
Spleen
Complementary DNA
Chromosomes
T-Lymphocytes
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Napolitano, M., Zingoni, A., Bernardini, G., Spinetti, G., Nista, A., Storlazzi, C. T., ... Santoni, A. (1996). Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues. Journal of Immunology, 157(7), 2759-2763.

Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues. / Napolitano, Monica; Zingoni, Alessandra; Bernardini, Giovanni; Spinetti, Gaia; Nista, Anna; Storlazzi, Clelia T.; Rocchi, Mariano; Santoni, Angela.

In: Journal of Immunology, Vol. 157, No. 7, 01.10.1996, p. 2759-2763.

Research output: Contribution to journalArticle

Napolitano, M, Zingoni, A, Bernardini, G, Spinetti, G, Nista, A, Storlazzi, CT, Rocchi, M & Santoni, A 1996, 'Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues', Journal of Immunology, vol. 157, no. 7, pp. 2759-2763.
Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A, Storlazzi CT et al. Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues. Journal of Immunology. 1996 Oct 1;157(7):2759-2763.
Napolitano, Monica ; Zingoni, Alessandra ; Bernardini, Giovanni ; Spinetti, Gaia ; Nista, Anna ; Storlazzi, Clelia T. ; Rocchi, Mariano ; Santoni, Angela. / Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues. In: Journal of Immunology. 1996 ; Vol. 157, No. 7. pp. 2759-2763.
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AB - Several chemokine receptors have been cloned and shown to belong to a superfamily of seven transmembrane, G protein-coupled receptors. We report here the molecular cloning of TER1, a novel human chemokine receptor-like gene. The amino acid sequence deduced from the TER1 cDNA shows 43, 40, 40, and 39% identity to CCR4, CCR5, CCR1, and CCR2B β chemokine receptors, respectively. By the use of fluorescent in situ hybridization, we have mapped the TER1 gene to chromosome 3p21, clustered with other chemokine receptor genes. By Northern blot analysis, TER1 mRNA is found to be expressed in the thymus, spleen, and at barely detectable levels in peripheral blood lymphocytes. Moreover, TER1 message is abundant in the NK cell line NK3.3 and in the T cell line MOLT-4. The restricted TER1 expression in cells and tissues of the lymphoid lineage suggests that this receptor may play a role in regulating immune functions.

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