Abstract
Several chemokine receptors have been cloned and shown to belong to a superfamily of seven transmembrane, G protein-coupled receptors. We report here the molecular cloning of TER1, a novel human chemokine receptor-like gene. The amino acid sequence deduced from the TER1 cDNA shows 43, 40, 40, and 39% identity to CCR4, CCR5, CCR1, and CCR2B β chemokine receptors, respectively. By the use of fluorescent in situ hybridization, we have mapped the TER1 gene to chromosome 3p21, clustered with other chemokine receptor genes. By Northern blot analysis, TER1 mRNA is found to be expressed in the thymus, spleen, and at barely detectable levels in peripheral blood lymphocytes. Moreover, TER1 message is abundant in the NK cell line NK3.3 and in the T cell line MOLT-4. The restricted TER1 expression in cells and tissues of the lymphoid lineage suggests that this receptor may play a role in regulating immune functions.
| Original language | English |
|---|---|
| Pages (from-to) | 2759-2763 |
| Number of pages | 5 |
| Journal | Journal of Immunology |
| Volume | 157 |
| Issue number | 7 |
| Publication status | Published - Oct 1 1996 |
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- Immunology
Cite this
Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues. / Napolitano, Monica; Zingoni, Alessandra; Bernardini, Giovanni; Spinetti, Gaia; Nista, Anna; Storlazzi, Clelia T.; Rocchi, Mariano; Santoni, Angela.
In: Journal of Immunology, Vol. 157, No. 7, 01.10.1996, p. 2759-2763.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular Cloning of TER1, a Chemokine Receptor-Like Gene Expressed by Lymphoid Tissues
AU - Napolitano, Monica
AU - Zingoni, Alessandra
AU - Bernardini, Giovanni
AU - Spinetti, Gaia
AU - Nista, Anna
AU - Storlazzi, Clelia T.
AU - Rocchi, Mariano
AU - Santoni, Angela
PY - 1996/10/1
Y1 - 1996/10/1
N2 - Several chemokine receptors have been cloned and shown to belong to a superfamily of seven transmembrane, G protein-coupled receptors. We report here the molecular cloning of TER1, a novel human chemokine receptor-like gene. The amino acid sequence deduced from the TER1 cDNA shows 43, 40, 40, and 39% identity to CCR4, CCR5, CCR1, and CCR2B β chemokine receptors, respectively. By the use of fluorescent in situ hybridization, we have mapped the TER1 gene to chromosome 3p21, clustered with other chemokine receptor genes. By Northern blot analysis, TER1 mRNA is found to be expressed in the thymus, spleen, and at barely detectable levels in peripheral blood lymphocytes. Moreover, TER1 message is abundant in the NK cell line NK3.3 and in the T cell line MOLT-4. The restricted TER1 expression in cells and tissues of the lymphoid lineage suggests that this receptor may play a role in regulating immune functions.
AB - Several chemokine receptors have been cloned and shown to belong to a superfamily of seven transmembrane, G protein-coupled receptors. We report here the molecular cloning of TER1, a novel human chemokine receptor-like gene. The amino acid sequence deduced from the TER1 cDNA shows 43, 40, 40, and 39% identity to CCR4, CCR5, CCR1, and CCR2B β chemokine receptors, respectively. By the use of fluorescent in situ hybridization, we have mapped the TER1 gene to chromosome 3p21, clustered with other chemokine receptor genes. By Northern blot analysis, TER1 mRNA is found to be expressed in the thymus, spleen, and at barely detectable levels in peripheral blood lymphocytes. Moreover, TER1 message is abundant in the NK cell line NK3.3 and in the T cell line MOLT-4. The restricted TER1 expression in cells and tissues of the lymphoid lineage suggests that this receptor may play a role in regulating immune functions.
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M3 - Article
C2 - 8816377
AN - SCOPUS:0030267843
VL - 157
SP - 2759
EP - 2763
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 7
ER -