Molecular cloning of the first human monoclona antibodies neutralizing with high potency Swine-origin Influenza A pandemic virus (S-OIV)

Roberto Burioni, Filippo Canducci, Nicasio Mancini, Nigcola Clementi, Monica Sassi, Donata De Marco, Diego Saita, Roberta Antonia Diotti, Giuseppe Sautto, Michela Sampaolo, Massimo Clementi

Research output: Contribution to journalArticle

Abstract

The pandemic caused by the new H1N1 swine-origin influenza virus (S-OIV) strain is a worldwide health emergency and alternative therapeutic and prophylactic options are greatly needed. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza strain at very low concentrations were cloned from a patient who had a broad-range anti-H1N1 serum neutralizing activity. The two HMabs neutralized S-OIV with an IC50 of 2.8 and 4 μg/mL. The genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered with the potentially of representing a novel drug to be used in the prophylaxis and the treatment of this human infection. This is the first report of molecular cloning of human monoclonal antibodies against the new pandemic swine-origin influenza virus.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalNew Microbiologica
Volume32
Issue number4
Publication statusPublished - Oct 2009

Keywords

  • Human monoclonal antibodies
  • Influenza pandemic
  • Swine influenza

ASJC Scopus subject areas

  • Microbiology (medical)

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    Burioni, R., Canducci, F., Mancini, N., Clementi, N., Sassi, M., De Marco, D., Saita, D., Diotti, R. A., Sautto, G., Sampaolo, M., & Clementi, M. (2009). Molecular cloning of the first human monoclona antibodies neutralizing with high potency Swine-origin Influenza A pandemic virus (S-OIV). New Microbiologica, 32(4), 319-324.