Molecular cloning of the mouse Ltbp-1 gene reveals tissue specific expression of alternatively spliced forms

Irene Noguera, Hiroto Obata, Anna Gualandris, Pamela Cowin, Daniel B. Rifkin

Research output: Contribution to journalArticle

Abstract

Latent transforming growth factor binding proteins (Ltbp-1, -2, -3 and -4) and fibrillins (Fbn-1 and -2) are structurally related cysteine-rich extracellular matrix proteins that localize to the 10 nm microfibrils. Ltbp-1 is thought to promote the secretion and proper folding of the small latent transforming growth factor β (TGF-β) complex (TGF-β plus its propeptide) and is implicated in sequestering it in the extracellular matrix. Here we report the isolation of the mouse Ltbp-1 complementary DNA (cDNA) and gene. The longer form of the Ltbp-1 cDNA encodes a predicted 1713 amino acid protein containing 18 epidermal growth factor-like repeats, four 8-cysteine domains and several motifs that suggest interactions with αIVβ1 and α9β1 integrins. Northern blotting analyses indicate that long and short Ltbp-1 transcripts are widely expressed in adult mouse tissues and most abundantly expressed in heart. Ltbp-1 is a single copy gene that maps to chromosome 17, band E (1-3) and encompasses more than 212 kb. The Ltbp-1 gene contains 34 exons and shows a similar organization to the LTBP-2 gene, suggesting that these genes originated from a common ancestral gene.

Original languageEnglish
Pages (from-to)31-41
Number of pages11
JournalGene
Volume308
Issue number1-2
DOIs
Publication statusPublished - Apr 10 2003

Fingerprint

Molecular Cloning
Transforming Growth Factors
Genes
Cysteine
Complementary DNA
Microfibrils
Chromosomes, Human, Pair 17
Extracellular Matrix Proteins
Epidermal Growth Factor
Integrins
Northern Blotting
Extracellular Matrix
Exons
Carrier Proteins
Amino Acids
Proteins

Keywords

  • Latent transforming growth factor β binding protein-1
  • Splice variants
  • Transforming growth factor β

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular cloning of the mouse Ltbp-1 gene reveals tissue specific expression of alternatively spliced forms. / Noguera, Irene; Obata, Hiroto; Gualandris, Anna; Cowin, Pamela; Rifkin, Daniel B.

In: Gene, Vol. 308, No. 1-2, 10.04.2003, p. 31-41.

Research output: Contribution to journalArticle

Noguera, Irene ; Obata, Hiroto ; Gualandris, Anna ; Cowin, Pamela ; Rifkin, Daniel B. / Molecular cloning of the mouse Ltbp-1 gene reveals tissue specific expression of alternatively spliced forms. In: Gene. 2003 ; Vol. 308, No. 1-2. pp. 31-41.
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