Molecular cloning of the mouse Ltbp-1 gene reveals tissue specific expression of alternatively spliced forms

Irene Noguera, Hiroto Obata, Anna Gualandris, Pamela Cowin, Daniel B. Rifkin

Research output: Contribution to journalArticle

Abstract

Latent transforming growth factor binding proteins (Ltbp-1, -2, -3 and -4) and fibrillins (Fbn-1 and -2) are structurally related cysteine-rich extracellular matrix proteins that localize to the 10 nm microfibrils. Ltbp-1 is thought to promote the secretion and proper folding of the small latent transforming growth factor β (TGF-β) complex (TGF-β plus its propeptide) and is implicated in sequestering it in the extracellular matrix. Here we report the isolation of the mouse Ltbp-1 complementary DNA (cDNA) and gene. The longer form of the Ltbp-1 cDNA encodes a predicted 1713 amino acid protein containing 18 epidermal growth factor-like repeats, four 8-cysteine domains and several motifs that suggest interactions with αIVβ1 and α9β1 integrins. Northern blotting analyses indicate that long and short Ltbp-1 transcripts are widely expressed in adult mouse tissues and most abundantly expressed in heart. Ltbp-1 is a single copy gene that maps to chromosome 17, band E (1-3) and encompasses more than 212 kb. The Ltbp-1 gene contains 34 exons and shows a similar organization to the LTBP-2 gene, suggesting that these genes originated from a common ancestral gene.

Original languageEnglish
Pages (from-to)31-41
Number of pages11
JournalGene
Volume308
Issue number1-2
DOIs
Publication statusPublished - Apr 10 2003

Keywords

  • Latent transforming growth factor β binding protein-1
  • Splice variants
  • Transforming growth factor β

ASJC Scopus subject areas

  • Genetics

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