Molecular cytogenetic characterization of stem-like cancer cells isolated from established cell lines

Patrizia Gasparini, Giulia Bertolini, Mara Binda, Alessandra Magnifico, Luisa Albano, Monica Tortoreto, Graziella Pratesi, Federica Facchinetti, Gabriella Abolafio, Luca Roz, Elda Tagliabue, Maria Grazia Daidone, Gabriella Sozzi

Research output: Contribution to journalArticle

Abstract

There is indication that tumor growth is sustained by subpopulation of cells with stem-like features but little is known on their genomic characterization and their genetic stability. We report a detailed molecular cytogenetic characterization using Spectral Karyotyping and fluorescent in situ hybridization of parental serum-cultured adherent cells and their sphere-growing stem-like counterpart before and after differentiation from six cell lines established from solid tumors. Our findings indicate increased cytogenetic complexity in sphere-growing stem-like and their differentiated adherent cells compared to parental adherent component suggesting the existence within cell lines of heterogeneous and genetically unstable subpopulations of cells endowed with stem-like features.

Original languageEnglish
Pages (from-to)206-215
Number of pages10
JournalCancer Letters
Volume296
Issue number2
DOIs
Publication statusPublished - Oct 2010

Keywords

  • Cancer stem cells
  • Cell lines
  • Chromosomal rearrangements
  • FISH
  • SKY

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Gasparini, P., Bertolini, G., Binda, M., Magnifico, A., Albano, L., Tortoreto, M., Pratesi, G., Facchinetti, F., Abolafio, G., Roz, L., Tagliabue, E., Daidone, M. G., & Sozzi, G. (2010). Molecular cytogenetic characterization of stem-like cancer cells isolated from established cell lines. Cancer Letters, 296(2), 206-215. https://doi.org/10.1016/j.canlet.2010.04.009