Molecular definition of group 1 innate lymphoid cells in the mouse uterus

Iva Filipovic, Laura Chiossone, Paola Vacca, Russell S Hamilton, Tiziano Ingegnere, Jean-Marc Doisne, Delia A Hawkes, Maria Cristina Mingari, Andrew M Sharkey, Lorenzo Moretta, Francesco Colucci

Research output: Contribution to journalArticle

Abstract

Determining the function of uterine lymphocytes is challenging because of the dynamic changes in response to sex hormones and, during pregnancy, to the invading foetal trophoblast cells. Here we provide a genome-wide transcriptome atlas of mouse uterine group 1 innate lymphoid cells (ILCs) at mid-gestation. Tissue-resident Eomes+CD49a+ NK cells (trNK), which resemble human uterine NK cells, are most abundant during early pregnancy, and have gene signatures associated with TGF-β responses and interactions with trophoblast, epithelial, endothelial, smooth muscle cells, leucocytes and extracellular matrix. Conventional NK cells expand late in gestation and may engage in crosstalk with trNK cells involving IL-18 and IFN-γ. Eomes-CD49a+ ILC1s dominate before puberty, and specifically expand in second pregnancies when the expression of the memory cell marker CXCR6 is upregulated. These results identify trNK cells as the cellular hub of uterine group 1 ILCs, and mark CXCR6+ ILC1s as potential memory cells of pregnancy.

Original languageEnglish
Pages (from-to)4492
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - Oct 29 2018

Fingerprint

uterus
Uterus
mice
Genes
Lymphocytes
Data storage equipment
Pregnancy
Interleukin-18
Gonadal Steroid Hormones
Crosstalk
cells
Natural Killer Cells
pregnancy
Muscle
Cells
Trophoblasts
Tissue
Atlases
Puberty
Transcriptome

Cite this

Molecular definition of group 1 innate lymphoid cells in the mouse uterus. / Filipovic, Iva; Chiossone, Laura; Vacca, Paola; Hamilton, Russell S; Ingegnere, Tiziano; Doisne, Jean-Marc; Hawkes, Delia A; Mingari, Maria Cristina; Sharkey, Andrew M; Moretta, Lorenzo; Colucci, Francesco.

In: Nature Communications, Vol. 9, No. 1, 29.10.2018, p. 4492.

Research output: Contribution to journalArticle

Filipovic, I, Chiossone, L, Vacca, P, Hamilton, RS, Ingegnere, T, Doisne, J-M, Hawkes, DA, Mingari, MC, Sharkey, AM, Moretta, L & Colucci, F 2018, 'Molecular definition of group 1 innate lymphoid cells in the mouse uterus', Nature Communications, vol. 9, no. 1, pp. 4492. https://doi.org/10.1038/s41467-018-06918-3
Filipovic, Iva ; Chiossone, Laura ; Vacca, Paola ; Hamilton, Russell S ; Ingegnere, Tiziano ; Doisne, Jean-Marc ; Hawkes, Delia A ; Mingari, Maria Cristina ; Sharkey, Andrew M ; Moretta, Lorenzo ; Colucci, Francesco. / Molecular definition of group 1 innate lymphoid cells in the mouse uterus. In: Nature Communications. 2018 ; Vol. 9, No. 1. pp. 4492.
@article{779dafdedd844130864dba06c6194e17,
title = "Molecular definition of group 1 innate lymphoid cells in the mouse uterus",
abstract = "Determining the function of uterine lymphocytes is challenging because of the dynamic changes in response to sex hormones and, during pregnancy, to the invading foetal trophoblast cells. Here we provide a genome-wide transcriptome atlas of mouse uterine group 1 innate lymphoid cells (ILCs) at mid-gestation. Tissue-resident Eomes+CD49a+ NK cells (trNK), which resemble human uterine NK cells, are most abundant during early pregnancy, and have gene signatures associated with TGF-β responses and interactions with trophoblast, epithelial, endothelial, smooth muscle cells, leucocytes and extracellular matrix. Conventional NK cells expand late in gestation and may engage in crosstalk with trNK cells involving IL-18 and IFN-γ. Eomes-CD49a+ ILC1s dominate before puberty, and specifically expand in second pregnancies when the expression of the memory cell marker CXCR6 is upregulated. These results identify trNK cells as the cellular hub of uterine group 1 ILCs, and mark CXCR6+ ILC1s as potential memory cells of pregnancy.",
author = "Iva Filipovic and Laura Chiossone and Paola Vacca and Hamilton, {Russell S} and Tiziano Ingegnere and Jean-Marc Doisne and Hawkes, {Delia A} and Mingari, {Maria Cristina} and Sharkey, {Andrew M} and Lorenzo Moretta and Francesco Colucci",
year = "2018",
month = "10",
day = "29",
doi = "10.1038/s41467-018-06918-3",
language = "English",
volume = "9",
pages = "4492",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "NLM (Medline)",
number = "1",

}

TY - JOUR

T1 - Molecular definition of group 1 innate lymphoid cells in the mouse uterus

AU - Filipovic, Iva

AU - Chiossone, Laura

AU - Vacca, Paola

AU - Hamilton, Russell S

AU - Ingegnere, Tiziano

AU - Doisne, Jean-Marc

AU - Hawkes, Delia A

AU - Mingari, Maria Cristina

AU - Sharkey, Andrew M

AU - Moretta, Lorenzo

AU - Colucci, Francesco

PY - 2018/10/29

Y1 - 2018/10/29

N2 - Determining the function of uterine lymphocytes is challenging because of the dynamic changes in response to sex hormones and, during pregnancy, to the invading foetal trophoblast cells. Here we provide a genome-wide transcriptome atlas of mouse uterine group 1 innate lymphoid cells (ILCs) at mid-gestation. Tissue-resident Eomes+CD49a+ NK cells (trNK), which resemble human uterine NK cells, are most abundant during early pregnancy, and have gene signatures associated with TGF-β responses and interactions with trophoblast, epithelial, endothelial, smooth muscle cells, leucocytes and extracellular matrix. Conventional NK cells expand late in gestation and may engage in crosstalk with trNK cells involving IL-18 and IFN-γ. Eomes-CD49a+ ILC1s dominate before puberty, and specifically expand in second pregnancies when the expression of the memory cell marker CXCR6 is upregulated. These results identify trNK cells as the cellular hub of uterine group 1 ILCs, and mark CXCR6+ ILC1s as potential memory cells of pregnancy.

AB - Determining the function of uterine lymphocytes is challenging because of the dynamic changes in response to sex hormones and, during pregnancy, to the invading foetal trophoblast cells. Here we provide a genome-wide transcriptome atlas of mouse uterine group 1 innate lymphoid cells (ILCs) at mid-gestation. Tissue-resident Eomes+CD49a+ NK cells (trNK), which resemble human uterine NK cells, are most abundant during early pregnancy, and have gene signatures associated with TGF-β responses and interactions with trophoblast, epithelial, endothelial, smooth muscle cells, leucocytes and extracellular matrix. Conventional NK cells expand late in gestation and may engage in crosstalk with trNK cells involving IL-18 and IFN-γ. Eomes-CD49a+ ILC1s dominate before puberty, and specifically expand in second pregnancies when the expression of the memory cell marker CXCR6 is upregulated. These results identify trNK cells as the cellular hub of uterine group 1 ILCs, and mark CXCR6+ ILC1s as potential memory cells of pregnancy.

U2 - 10.1038/s41467-018-06918-3

DO - 10.1038/s41467-018-06918-3

M3 - Article

C2 - 30374017

VL - 9

SP - 4492

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -