Molecular Detection of Human Salivirus in Italy Through Monitoring of Urban Sewages

Pamela Mancini, Giusy Bonanno Ferraro, Elisabetta Suffredini, Carolina Veneri, Marcello Iaconelli, Teresa Vicenza, Giuseppina La Rosa

Research output: Contribution to journalArticle

Abstract

Salivirus (SalV) is a newly discovered virus associated to acute gastroenteritis in humans. In Italy, its prevalence and genetic diversity is unknown. To reduce this knowledge gap, 124 sewage samples collected throughout the country were analyzed for SalV by two nested RT-PCRs targeting the 5'UTR and the 3D regions and by real-time RT-qPCR. Virus RNA was detected in 37 (29.8%) samples; of these, 24 could be characterized and all belonged to genotype A1. Viral concentrations ranged between 2.8 × 103 and 1.9 × 105 genome copies per liter. This is the first report of SalV occurrence in water environments in Italy, suggesting that SalV infection is not uncommon in this country.

Original languageEnglish
Pages (from-to)68-74
Number of pages7
JournalFood and Environmental Virology
Volume12
Issue number1
DOIs
Publication statusPublished - Mar 2020

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