Molecular detection of neuron-specific ELAV-like-positive cells in the peripheral blood of patients with small-cell lung cancer

Vito D'Alessandro, Lucia Anna Muscarella, Massimiliano Copetti, Leopoldo Zelante, Massimo Carella, Gianluigi Vendemiale

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: n-ELAV (neuronal-Embryonic Lethal, Abnormal Vision)-like genes belong to a family codifying for onconeural RNA-binding proteins. Anti-Hu-antibodies (anti-Hu-Ab) are typically associated with paraneoplastic encephalomyelitis/sensory neuropathy (PEM/PSN), and low titres of anti-Hu-Ab, were found in newly diagnosed Small Cell Lung Cancer (SCLC). The aim of this study is to develop a sensitive and quantitative molecular real-time PCR assay to detect SCLC cells in peripheral blood (PB) through nELAV-like transcripts quantification. Methods: Peripheral blood samples from 25 SCLC untreated patients and 12 healthy blood donors were investigated by real-time PCR. mRNA levels for HuB (ELAV2), HuC (ELAV3) and HuD (ELAV4) were measured in peripheral blood samples with an absolute quantification method using plasmid dilutions as calibration curves. Results: A statistically significant increase in mRNA expression level was detected for HuB and HuD in SCLC patients as compared with samples from healthy blood donors. After establishing cut off values based on the level of expression in control samples, 28% of the SCLC samples were positive for HuD expression. Overall 60% of the SCLC displayed increased level of HuD or HuB transcripts. Conclusion: Our preliminary results suggest that neuron-ELAV mRNA are detectable in peripheral blood of SCLC patients using real-time quantitative PCR.

Original languageEnglish
Pages (from-to)291-297
Number of pages7
JournalCellular Oncology
Volume30
Issue number4
DOIs
Publication statusPublished - 2008

Fingerprint

Small Cell Lung Carcinoma
Neurons
Real-Time Polymerase Chain Reaction
Blood Donors
Messenger RNA
Anti-Idiotypic Antibodies
Nervous System Paraneoplastic Syndromes
RNA-Binding Proteins
Calibration
Blood Cells
Plasmids
Genes

Keywords

  • ELAV-like
  • Plasmid dilutions
  • Real-time quantitative PCR
  • SCLC

ASJC Scopus subject areas

  • Cell Biology
  • Pathology and Forensic Medicine
  • Oncology

Cite this

Molecular detection of neuron-specific ELAV-like-positive cells in the peripheral blood of patients with small-cell lung cancer. / D'Alessandro, Vito; Muscarella, Lucia Anna; Copetti, Massimiliano; Zelante, Leopoldo; Carella, Massimo; Vendemiale, Gianluigi.

In: Cellular Oncology, Vol. 30, No. 4, 2008, p. 291-297.

Research output: Contribution to journalArticle

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AU - Zelante, Leopoldo

AU - Carella, Massimo

AU - Vendemiale, Gianluigi

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N2 - Background: n-ELAV (neuronal-Embryonic Lethal, Abnormal Vision)-like genes belong to a family codifying for onconeural RNA-binding proteins. Anti-Hu-antibodies (anti-Hu-Ab) are typically associated with paraneoplastic encephalomyelitis/sensory neuropathy (PEM/PSN), and low titres of anti-Hu-Ab, were found in newly diagnosed Small Cell Lung Cancer (SCLC). The aim of this study is to develop a sensitive and quantitative molecular real-time PCR assay to detect SCLC cells in peripheral blood (PB) through nELAV-like transcripts quantification. Methods: Peripheral blood samples from 25 SCLC untreated patients and 12 healthy blood donors were investigated by real-time PCR. mRNA levels for HuB (ELAV2), HuC (ELAV3) and HuD (ELAV4) were measured in peripheral blood samples with an absolute quantification method using plasmid dilutions as calibration curves. Results: A statistically significant increase in mRNA expression level was detected for HuB and HuD in SCLC patients as compared with samples from healthy blood donors. After establishing cut off values based on the level of expression in control samples, 28% of the SCLC samples were positive for HuD expression. Overall 60% of the SCLC displayed increased level of HuD or HuB transcripts. Conclusion: Our preliminary results suggest that neuron-ELAV mRNA are detectable in peripheral blood of SCLC patients using real-time quantitative PCR.

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