Molecular detection of TP53, Ki-Ras and p16INK4A promoter methylation in plasma of patients with colorectal cancer and its association with prognosis. Results of a 3-year GOIM (Gruppo Oncologico dell'Italia Meridionale) prospective study

V. Bazan, L. Bruno, C. Augello, V. Agnese, V. Calò, S. Corsale, G. Gargano, M. Terrasi, V. Schirò, G. Di Fede, V. Adamo, C. Intrivici, A. Crosta, G. Rinaldi, F. Latteri, G. Dardanoni, N. Grassi, M. R. Valerio, G. Colucci, M. MacalusoA. Russo

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Despite the improvement in detection and surgical therapy in the last years, the outcome of patients affected by colorectal carcinoma (CRC) remains limited by metastatic relapse. The aim of this study was to investigate the presence of free tumor DNA in the plasma of CRC patients in order to understand its possible prognostic role. Patients and methods: Ki-Ras, TP53 mutations and p16INK4A methylation status were prospectively evaluated in tumor tissues and plasma of 66 CRC patients. Results: In 50 of the 66 primitive tumor cases (76%) at least one significant alteration was identified in Ki-Ras and/or TP53 and/or p16INK4A genes. Eighteen of the 50 patients presented the same alteration both in the plasma and in the tumor tissue. At univariate analysis, Ki-Ras mutations proved to be significantly related to quicker relapse (P

Original languageEnglish
JournalAnnals of Oncology
Volume17
Issue numberSUPPL. 7
DOIs
Publication statusPublished - Jun 2006

Keywords

  • Colorectal carcinoma
  • Free-cell DNA
  • Ki-Ras
  • TP53

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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