Molecular diagnosis of a mycobacterium chelonae infection

Stephan Schulz, Markus Kremer, Antonello D. Cabras, Andreas Lügering, Matthias Seidel, Heinz Höfler, Martin Werner

Research output: Contribution to journalArticle

Abstract

A 23-year-old female presented with enlarged cervical lymph nodes, and a diagnosis of nonspecific lymphadenitis with formation of pyogranulomas was rendered. Despite an initial oral antibiosis and subsequent long-term intravenous and oral antibiosis under hospitalized conditions, the symptoms progressed. The lymph nodes became larger and then affected the cervical region bilaterally. Her general condition worsened, and an exanthema of the extremities accompanied by a reactive arthritis occurred. Serological assays of various viral and bacterial markers and blood cultures were negative. Application of a polymerase chain reaction (PCR) protocol allowing specific amplification of mycobacterial DNA revealed DNA of Mycobacterium chelonea in formalin-fixed, paraffin-embedded lymph node tissue. Sequencing of the PCR product showed a 97% homology with the known Mycobacterium chelonae sequence. Modification of the antibiotic therapy with clarithromycin, imipenem and amikacin resulted in a rapid regression of the symptoms. The clinical course, in combination with the difficulties in detecting the infectious agent, supports the usefulness of molecular pathological analyses specific for nontuberculous mycobacteria (NTM).

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalPathology Research and Practice
Volume197
Issue number2
Publication statusPublished - 2001

Fingerprint

Mycobacterium chelonae
Mycobacterium Infections
Antibiosis
Lymph Nodes
Nontuberculous Mycobacteria
Reactive Arthritis
Polymerase Chain Reaction
Lymphadenitis
Clarithromycin
Amikacin
Imipenem
DNA
Mycobacterium
Exanthema
Paraffin
Formaldehyde
Extremities
Biomarkers
Anti-Bacterial Agents
Therapeutics

Keywords

  • Antituberculosis drugs
  • Cervical lymphadenitis
  • Molecular pathology
  • Mycobacterium chelonae
  • Nontuberculous mycobacteria

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Schulz, S., Kremer, M., Cabras, A. D., Lügering, A., Seidel, M., Höfler, H., & Werner, M. (2001). Molecular diagnosis of a mycobacterium chelonae infection. Pathology Research and Practice, 197(2), 123-126.

Molecular diagnosis of a mycobacterium chelonae infection. / Schulz, Stephan; Kremer, Markus; Cabras, Antonello D.; Lügering, Andreas; Seidel, Matthias; Höfler, Heinz; Werner, Martin.

In: Pathology Research and Practice, Vol. 197, No. 2, 2001, p. 123-126.

Research output: Contribution to journalArticle

Schulz, S, Kremer, M, Cabras, AD, Lügering, A, Seidel, M, Höfler, H & Werner, M 2001, 'Molecular diagnosis of a mycobacterium chelonae infection', Pathology Research and Practice, vol. 197, no. 2, pp. 123-126.
Schulz S, Kremer M, Cabras AD, Lügering A, Seidel M, Höfler H et al. Molecular diagnosis of a mycobacterium chelonae infection. Pathology Research and Practice. 2001;197(2):123-126.
Schulz, Stephan ; Kremer, Markus ; Cabras, Antonello D. ; Lügering, Andreas ; Seidel, Matthias ; Höfler, Heinz ; Werner, Martin. / Molecular diagnosis of a mycobacterium chelonae infection. In: Pathology Research and Practice. 2001 ; Vol. 197, No. 2. pp. 123-126.
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