Molecular diagnosis of p53 mutations in gastric carcinoma by touch preparation

Enrico Ricevuto, Corrado Ficorella, Carlo Fusco, Katia Cannita, Alessandra Tessitore, Elena Toniato, Angela Gabriele, Luigi Frati, Paolo Marchetti, Alberto Gulino, Stefano Martinotti

Research output: Contribution to journalArticle

Abstract

Thirty-one tumor samples from selected cases of gastric carcinoma were analyzed for mutations of the p53 tumor suppressor gene. Template DNA was prepared according to the touch preparation procedure, which allowed us to isolate clusters of neoplastic cells out of a stromal cellular background to be used as a template in the amplification of target exons of the p53 locus. In our present study, by polymerase chain reaction/single strand conformation polymorphism analysis we give evidence of p53 mutations occurring in the DNA- binding core domain of the protein (exons 5 through 9), which are clustered in stages III and IV of the disease (six mutations out of seventeen samples; 35%). No p53 mutations were detected in fourteen gastric cancer samples at I and II stages. Beside the use of conventional molecular scanning procedures, our study proposes the application of the touch preparation method to increase the detection of genetic alterations in human solid tumors.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalAmerican Journal of Pathology
Volume148
Issue number2
Publication statusPublished - Feb 1996

    Fingerprint

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ricevuto, E., Ficorella, C., Fusco, C., Cannita, K., Tessitore, A., Toniato, E., Gabriele, A., Frati, L., Marchetti, P., Gulino, A., & Martinotti, S. (1996). Molecular diagnosis of p53 mutations in gastric carcinoma by touch preparation. American Journal of Pathology, 148(2), 405-413.