Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish

Viviana E. Gallardo, Jin Liang, Martine Behra, Abdel Elkahloun, Eduardo J. Villablanca, Vincenzo Russo, Miguel L. Allende, Shawn M. Burgess

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background. Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that traverses the length of the fish to form this sensory system. As it migrates, the primordium deposits groups of cells called neuromasts, the specialized organs that contain the mechanosensory hair cells. Therefore the primordium provides both a model for studying collective directional cell migration and the differentiation of sensory cells from multipotent progenitor cells. Results. Through the combined use of transgenic fish, Fluorescence Activated Cell Sorting and microarray analysis we identified a repertoire of key genes expressed in the migrating primordium and in differentiated neuromasts. We validated the specific expression in the primordium of a subset of the identified sequences by quantitative RT-PCR, and by in situ hybridization. We also show that interfering with the function of two genes, f11r and cd9b, defects in primordium migration are induced. Finally, pathway construction revealed functional relationships among the genes enriched in the migrating cell population. Conclusions. Our results demonstrate that this is a robust approach to globally analyze tissue-specific expression and we predict that many of the genes identified in this study will show critical functions in developmental events involving collective cell migration and possibly in pathological situations such as tumor metastasis.

Original languageEnglish
Article number120
JournalBMC Developmental Biology
Volume10
DOIs
Publication statusPublished - 2010

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Zebrafish
Dissection
Cell Movement
Genes
Cell Differentiation
Fishes
Lateral Line System
Tissue Array Analysis
In Situ Hybridization
Flow Cytometry
Stem Cells
Cell Proliferation
Neoplasm Metastasis
Polymerase Chain Reaction
Population
Neoplasms

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish. / Gallardo, Viviana E.; Liang, Jin; Behra, Martine; Elkahloun, Abdel; Villablanca, Eduardo J.; Russo, Vincenzo; Allende, Miguel L.; Burgess, Shawn M.

In: BMC Developmental Biology, Vol. 10, 120, 2010.

Research output: Contribution to journalArticle

Gallardo, Viviana E. ; Liang, Jin ; Behra, Martine ; Elkahloun, Abdel ; Villablanca, Eduardo J. ; Russo, Vincenzo ; Allende, Miguel L. ; Burgess, Shawn M. / Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish. In: BMC Developmental Biology. 2010 ; Vol. 10.
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