Molecular epidemiology and genotyping of Mycobacterium tuberculosis isolated in Baghdad

Ruqaya Mustafa Ali, Alberto Trovato, David Couvin, Amina N. Al-Thwani, Emanuele Borroni, Fahim H. Dhaer, Nalin Rastogi, Daniela M. Cirillo

Research output: Contribution to journalArticle

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Abstract

Tuberculosis (TB) remains a major health problem in Iraq but the strains responsible for the epidemic have been poorly characterized. Our aim was to characterize the TB strains circulating in Bagdad (Iraq). A total of 270 Mycobacterium tuberculosis complex (MTBC) strains isolated between 2010 and 2011 from TB patients attending the Center of Chest and Respiratory diseases in Baghdad were analyzed by Spoligotyping. The analysis indicated that 94.1% of the isolates belong to known genotype clades: CAS 39.6%, ill-defined T clade 29.6%, Manu 7.4%, Haarlem 7%, Ural 4.1%, LAM 3.3%, X 0.7%, LAM7-TUR 0.7%, EAI 0.7%, S 0.7%, and unknown 5.9%. Comparison with the international multimarker database SITVIT2 showed that SIT 309 (CAS1-Delhi) and SIT1144 (T1) were the most common types. In addition, 44 strains were included in SITVIT2 database under 16 new Spoligotype International Types (SITs); of these, 6 SITs (SIT3346, SIT3497, SIT3708, SIT3790, SIT3791, and SIT3800) (n = 32 strains) were created within the present study and 10 were created after a match with an orphan in the database. By using 24-loci MIRU-VNTR-typing on a subset of 110 samples we found a high recent transmission index (RTI) of 33.6%. In conclusion, we present the first unifying framework for both epidemiology and evolutionary analysis of M. tuberculosis in Iraq.

Original languageEnglish
Article number580981
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

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Iraq
Molecular Epidemiology
Mycobacterium tuberculosis
Tuberculosis
Databases
Respiratory Center
Epidemiology
Pulmonary diseases
Minisatellite Repeats
Orphaned Children
Medical problems
Thorax
Genotype
Health

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Molecular epidemiology and genotyping of Mycobacterium tuberculosis isolated in Baghdad. / Mustafa Ali, Ruqaya; Trovato, Alberto; Couvin, David; Al-Thwani, Amina N.; Borroni, Emanuele; Dhaer, Fahim H.; Rastogi, Nalin; Cirillo, Daniela M.

In: BioMed Research International, Vol. 2014, 580981, 2014.

Research output: Contribution to journalArticle

Mustafa Ali, Ruqaya ; Trovato, Alberto ; Couvin, David ; Al-Thwani, Amina N. ; Borroni, Emanuele ; Dhaer, Fahim H. ; Rastogi, Nalin ; Cirillo, Daniela M. / Molecular epidemiology and genotyping of Mycobacterium tuberculosis isolated in Baghdad. In: BioMed Research International. 2014 ; Vol. 2014.
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