Molecular epidemiology of JCV genotypes in patients and healthy subjects from Northern Italy

Nunzia Zanotta, Serena Delbue, Tatiana Rossi, Giorgio Pelos, Pierlanfranco D'Agaro, Lorenzo Monasta, Pasquale Ferrante, Manola Comar

Research output: Contribution to journalArticlepeer-review

Abstract

Very little is known about JCV infection and genotype distribution with respect to the different demographic and clinical characteristics of the Italian population. A cross-sectional study was carried out on the prevalence of JCV genotypes in 323 Caucasian subjects (mean age: 37.5 years, range: 2-70 years). Urine samples from 200 immunocompromised patients, including patients affected by Multiple Sclerosis (MS), Human Immunodeficiency Virus (HIV), colon cancer, inflammatory diseases and Progressive Multifocal Leukoencephalopathy (PML), and 123 immunocompetent individuals were tested by quantitative real time PCR. Sequencing of the JCV viral protein 1 (VP1) and transcriptional control region (TCR) was performed. In this series, the overall prevalence of JCV excretion was 32.8% without significant differences between males and females. JCV was detected in 39.5% of patients and in 22% of healthy individuals (P=0.004). The most prevalent JCV genotype excreted was genotype 1 (69.8%), followed by genotype 2 (22.6%) and genotype 4 (7.5%). Distribution of genotypes between patients and healthy subjects showed a statistically significant difference for the type 1 compared to the other variants (P

Original languageEnglish
Pages (from-to)1286-1292
Number of pages7
JournalJournal of Medical Virology
Volume85
Issue number7
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Immunocompromised and healthy subjects
  • JCV
  • Subtypes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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