Molecular evidence for antigen drive in the natural history of mantle cell lymphoma

Aliki Xochelli, Lesley Ann Sutton, Andreas Agathangelidis, Evangelia Stalika, Maria Karypidou, Fotini Marantidou, Alba Navarro Lopez, Giorgos Papadopoulos, Jana Supikova, Patricia Groenen, Myriam Boudjogra, Christer Sundstrom, Maurilio Ponzoni, Hana Skuhrova Francova, Achilles Anagnostopoulos, Sarka Pospisilova, Theodora Papadaki, Dimitris Tzovaras, Paolo Ghia, Christiane PottFrederic Davi, Elias Campo, Richard Rosenquist, Anastasia Hadzidimitriou, Chrysoula Belessi, Kostas Stamatopoulos

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To further our understanding about antigen involvement in mantle cell lymphoma (MCL), we analyzed the expression levels of activation-induced cytidine deaminase (AID), a key player in B-cell responses to antigen triggering, in 133 MCL cases; assessed the functionality of AID by evaluating in vivo class switch recombination in 52 MCL cases; and sought for indications of ongoing antigen interactions by exploring intraclonal diversification within 14 MCL cases. The AID full-length transcript and the most frequent splice variants (AID-ΔE4a, AID-ΔE) were detected in 128 (96.2%), 96 (72.2%), and 130 cases (97.7%), respectively. Higher AID full-length transcript levels were significantly associated (P <0.001) with lack of somatic hypermutation within the clonotypic immunoglobulin heavy variable (IGHV) genes. Median AID transcript levels were higher in lymph node material compared to cases in which peripheral blood was analyzed, implying that clonal behavior is influenced by the microenvironment. Switched tumor-derived IGHV-IGHD-IGHJ transcripts were identified in 5 of 52 cases (9.6%), all of which displayed somatic hypermutation and AID-mRNA expression. Finally, although most cases exhibited low levels of intraclonal diversification, analysis of the mutational activity revealed a precise targeting of somatic hypermutation indicative of an active, ongoing interaction with antigen(s). Collectively, these findings strongly allude to antigen involvement in the natural history of MCL, further challenging the notion of antigen naivety.

Original languageEnglish
Pages (from-to)1740-1748
Number of pages9
JournalAmerican Journal of Pathology
Volume185
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

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Mantle-Cell Lymphoma
Antigens
Immunoglobulins
AICDA (activation-induced cytidine deaminase)
Genetic Recombination
B-Lymphocytes
Lymph Nodes
Messenger RNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Xochelli, A., Sutton, L. A., Agathangelidis, A., Stalika, E., Karypidou, M., Marantidou, F., ... Stamatopoulos, K. (2015). Molecular evidence for antigen drive in the natural history of mantle cell lymphoma. American Journal of Pathology, 185(6), 1740-1748. https://doi.org/10.1016/j.ajpath.2015.02.006

Molecular evidence for antigen drive in the natural history of mantle cell lymphoma. / Xochelli, Aliki; Sutton, Lesley Ann; Agathangelidis, Andreas; Stalika, Evangelia; Karypidou, Maria; Marantidou, Fotini; Lopez, Alba Navarro; Papadopoulos, Giorgos; Supikova, Jana; Groenen, Patricia; Boudjogra, Myriam; Sundstrom, Christer; Ponzoni, Maurilio; Francova, Hana Skuhrova; Anagnostopoulos, Achilles; Pospisilova, Sarka; Papadaki, Theodora; Tzovaras, Dimitris; Ghia, Paolo; Pott, Christiane; Davi, Frederic; Campo, Elias; Rosenquist, Richard; Hadzidimitriou, Anastasia; Belessi, Chrysoula; Stamatopoulos, Kostas.

In: American Journal of Pathology, Vol. 185, No. 6, 01.06.2015, p. 1740-1748.

Research output: Contribution to journalArticle

Xochelli, A, Sutton, LA, Agathangelidis, A, Stalika, E, Karypidou, M, Marantidou, F, Lopez, AN, Papadopoulos, G, Supikova, J, Groenen, P, Boudjogra, M, Sundstrom, C, Ponzoni, M, Francova, HS, Anagnostopoulos, A, Pospisilova, S, Papadaki, T, Tzovaras, D, Ghia, P, Pott, C, Davi, F, Campo, E, Rosenquist, R, Hadzidimitriou, A, Belessi, C & Stamatopoulos, K 2015, 'Molecular evidence for antigen drive in the natural history of mantle cell lymphoma', American Journal of Pathology, vol. 185, no. 6, pp. 1740-1748. https://doi.org/10.1016/j.ajpath.2015.02.006
Xochelli, Aliki ; Sutton, Lesley Ann ; Agathangelidis, Andreas ; Stalika, Evangelia ; Karypidou, Maria ; Marantidou, Fotini ; Lopez, Alba Navarro ; Papadopoulos, Giorgos ; Supikova, Jana ; Groenen, Patricia ; Boudjogra, Myriam ; Sundstrom, Christer ; Ponzoni, Maurilio ; Francova, Hana Skuhrova ; Anagnostopoulos, Achilles ; Pospisilova, Sarka ; Papadaki, Theodora ; Tzovaras, Dimitris ; Ghia, Paolo ; Pott, Christiane ; Davi, Frederic ; Campo, Elias ; Rosenquist, Richard ; Hadzidimitriou, Anastasia ; Belessi, Chrysoula ; Stamatopoulos, Kostas. / Molecular evidence for antigen drive in the natural history of mantle cell lymphoma. In: American Journal of Pathology. 2015 ; Vol. 185, No. 6. pp. 1740-1748.
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AU - Karypidou, Maria

AU - Marantidou, Fotini

AU - Lopez, Alba Navarro

AU - Papadopoulos, Giorgos

AU - Supikova, Jana

AU - Groenen, Patricia

AU - Boudjogra, Myriam

AU - Sundstrom, Christer

AU - Ponzoni, Maurilio

AU - Francova, Hana Skuhrova

AU - Anagnostopoulos, Achilles

AU - Pospisilova, Sarka

AU - Papadaki, Theodora

AU - Tzovaras, Dimitris

AU - Ghia, Paolo

AU - Pott, Christiane

AU - Davi, Frederic

AU - Campo, Elias

AU - Rosenquist, Richard

AU - Hadzidimitriou, Anastasia

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