Molecular evidence in support of the neoplastic and precursor nature of microglandular adenosis

Felipe C. Geyer, Magali Lacroix-Triki, Pierre Emmanuel Colombo, Neill Patani, Arnaud Gauthier, Rachael Natrajan, Maryou B K Lambros, Ibrahim Khalifeh, Constance Albarracin, Sandra Orru, Caterina Marchiò, Anna Sapino, Alan Mackay, Britta Weigelt, Fernando C. Schmitt, Jelle Wesseling, Nour Sneige, Jorge S. Reis-Filho

Research output: Contribution to journalArticle

Abstract

Aims: Microglandular adenosis (MGA) is a proliferative breast lesion, which has been proposed to be a potential precursor of triple-negative breast cancers. The aims of this study were to determine whether MGAs harbour genetic alterations and if any such genetic aberrations found in MGAs are similar to those found in matched invasive carcinomas. Methods and results: Twelve cases of MGA and/or atypical MGA (AMGA), 10 of which were associated with invasive carcinoma, were evaluated. Immunohistochemical profiling revealed that all invasive carcinomas were of triple-negative phenotype and expressed S100, cytokeratins 8/18 and 'basal' markers. The morphologically distinct components of each case (MGA, AMGA and/or invasive carcinoma) were microdissected and subjected to microarray comparative genomic hybridization. Apart from three typical MGAs, all samples harboured genetic alterations. The percentage of the genome affected by copy number aberrations in MGA/AMGA ranged from 0.5 to 61.9%, indicating varying levels of genetic instability. In three cases, MGA/AMGA displayed copy number aberrations similar to those found in matched invasive components, providing strong circumstantial evidence that MGA may constitute the substrate for the invasive carcinoma development. Conclusions: Our results support the contention that MGA can be a clonal lesion and non-obligate precursor of triple-negative breast cancer.

Original languageEnglish
JournalHistopathology
Volume60
Issue number6 B
DOIs
Publication statusPublished - May 2012

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Keywords

  • Basal-like
  • Comparative genomic hybridization
  • Immunohistochemistry
  • Invasive ductal carcinoma
  • Microglandular adenosis
  • Triple-negative

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Geyer, F. C., Lacroix-Triki, M., Colombo, P. E., Patani, N., Gauthier, A., Natrajan, R., Lambros, M. B. K., Khalifeh, I., Albarracin, C., Orru, S., Marchiò, C., Sapino, A., Mackay, A., Weigelt, B., Schmitt, F. C., Wesseling, J., Sneige, N., & Reis-Filho, J. S. (2012). Molecular evidence in support of the neoplastic and precursor nature of microglandular adenosis. Histopathology, 60(6 B). https://doi.org/10.1111/j.1365-2559.2012.04207.x