Molecular evidences for a role of HSV-1 in multiple sclerosis clinical acute attack

Pasquale Ferrante, Roberta Mancuso, Elisabetta Pagani, Franca Rosa Guerini, Maria Gaetana Calvo, Marina Saresella, Livianna Speciale, Domenico Caputo

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

To verify the possible role of human herpesviruses as triggering or aggravating factors in relapsing-remitting multiple sclerosis (RRMS) clinical acute attack, we studied the prevalence of some herpesviruses in the peripheral blood mononuclear cells (PBMCs) collected from 22 MS patients during an MS relapse and in a stable phase and from 18 healthy controls (HC). DNA belonging to Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and Human Herpes virus 6 (HHV-6) has been searched by specific nested polymerase chain reaction (n-PCR). EBV and HHV6 DNA has been detected with high frequency in acute and stable MS and in healthy controls without significant differences. HCMV DNA was observed both in acute and stable MS but not in HC, and, more interestingly, HSV-1 DNA was only found in 13% of acute MS, while both stable MS and healthy controls were negative. On the basis of these results we focused on HSV-1, and to confirm them and to demonstrate that HSV-1 is actively replicating in MS patients during clinical relapse, we searched both messenger RNA (mRNA) and DNA of HSV-1 in the PBMCs of 15 acute MS patients and 15 healthy controls. We found HSV-1 mRNA and DNA in a significant number of acute MS patients but not in the control group. On the whole these data indicate that HSV-1 reactivate in the peripheral blood of MS patients during clinical acute attack and probably play a role in the triggering of MS relapses.

Original languageEnglish
JournalJournal of NeuroVirology
Volume6
Issue numberSUPPL. 2
Publication statusPublished - 2000

Fingerprint

Human Herpesvirus 1
Multiple Sclerosis
DNA
Herpesviridae
Cytomegalovirus
Human Herpesvirus 4
Recurrence
Blood Cells
Human Herpesvirus 6
Relapsing-Remitting Multiple Sclerosis
Messenger RNA
Human Herpesvirus 2
Viruses
Polymerase Chain Reaction
Control Groups

Keywords

  • Clinical relapse
  • DNA
  • Herpes simplex type 1 and 2
  • Herpesviruses
  • Messenger RNA
  • Multiple sclerosis

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Molecular evidences for a role of HSV-1 in multiple sclerosis clinical acute attack. / Ferrante, Pasquale; Mancuso, Roberta; Pagani, Elisabetta; Guerini, Franca Rosa; Calvo, Maria Gaetana; Saresella, Marina; Speciale, Livianna; Caputo, Domenico.

In: Journal of NeuroVirology, Vol. 6, No. SUPPL. 2, 2000.

Research output: Contribution to journalArticle

Ferrante, Pasquale ; Mancuso, Roberta ; Pagani, Elisabetta ; Guerini, Franca Rosa ; Calvo, Maria Gaetana ; Saresella, Marina ; Speciale, Livianna ; Caputo, Domenico. / Molecular evidences for a role of HSV-1 in multiple sclerosis clinical acute attack. In: Journal of NeuroVirology. 2000 ; Vol. 6, No. SUPPL. 2.
@article{9a9caae27e62467c892955e7045de4c0,
title = "Molecular evidences for a role of HSV-1 in multiple sclerosis clinical acute attack",
abstract = "To verify the possible role of human herpesviruses as triggering or aggravating factors in relapsing-remitting multiple sclerosis (RRMS) clinical acute attack, we studied the prevalence of some herpesviruses in the peripheral blood mononuclear cells (PBMCs) collected from 22 MS patients during an MS relapse and in a stable phase and from 18 healthy controls (HC). DNA belonging to Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and Human Herpes virus 6 (HHV-6) has been searched by specific nested polymerase chain reaction (n-PCR). EBV and HHV6 DNA has been detected with high frequency in acute and stable MS and in healthy controls without significant differences. HCMV DNA was observed both in acute and stable MS but not in HC, and, more interestingly, HSV-1 DNA was only found in 13{\%} of acute MS, while both stable MS and healthy controls were negative. On the basis of these results we focused on HSV-1, and to confirm them and to demonstrate that HSV-1 is actively replicating in MS patients during clinical relapse, we searched both messenger RNA (mRNA) and DNA of HSV-1 in the PBMCs of 15 acute MS patients and 15 healthy controls. We found HSV-1 mRNA and DNA in a significant number of acute MS patients but not in the control group. On the whole these data indicate that HSV-1 reactivate in the peripheral blood of MS patients during clinical acute attack and probably play a role in the triggering of MS relapses.",
keywords = "Clinical relapse, DNA, Herpes simplex type 1 and 2, Herpesviruses, Messenger RNA, Multiple sclerosis",
author = "Pasquale Ferrante and Roberta Mancuso and Elisabetta Pagani and Guerini, {Franca Rosa} and Calvo, {Maria Gaetana} and Marina Saresella and Livianna Speciale and Domenico Caputo",
year = "2000",
language = "English",
volume = "6",
journal = "Journal of NeuroVirology",
issn = "1355-0284",
publisher = "Springer New York",
number = "SUPPL. 2",

}

TY - JOUR

T1 - Molecular evidences for a role of HSV-1 in multiple sclerosis clinical acute attack

AU - Ferrante, Pasquale

AU - Mancuso, Roberta

AU - Pagani, Elisabetta

AU - Guerini, Franca Rosa

AU - Calvo, Maria Gaetana

AU - Saresella, Marina

AU - Speciale, Livianna

AU - Caputo, Domenico

PY - 2000

Y1 - 2000

N2 - To verify the possible role of human herpesviruses as triggering or aggravating factors in relapsing-remitting multiple sclerosis (RRMS) clinical acute attack, we studied the prevalence of some herpesviruses in the peripheral blood mononuclear cells (PBMCs) collected from 22 MS patients during an MS relapse and in a stable phase and from 18 healthy controls (HC). DNA belonging to Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and Human Herpes virus 6 (HHV-6) has been searched by specific nested polymerase chain reaction (n-PCR). EBV and HHV6 DNA has been detected with high frequency in acute and stable MS and in healthy controls without significant differences. HCMV DNA was observed both in acute and stable MS but not in HC, and, more interestingly, HSV-1 DNA was only found in 13% of acute MS, while both stable MS and healthy controls were negative. On the basis of these results we focused on HSV-1, and to confirm them and to demonstrate that HSV-1 is actively replicating in MS patients during clinical relapse, we searched both messenger RNA (mRNA) and DNA of HSV-1 in the PBMCs of 15 acute MS patients and 15 healthy controls. We found HSV-1 mRNA and DNA in a significant number of acute MS patients but not in the control group. On the whole these data indicate that HSV-1 reactivate in the peripheral blood of MS patients during clinical acute attack and probably play a role in the triggering of MS relapses.

AB - To verify the possible role of human herpesviruses as triggering or aggravating factors in relapsing-remitting multiple sclerosis (RRMS) clinical acute attack, we studied the prevalence of some herpesviruses in the peripheral blood mononuclear cells (PBMCs) collected from 22 MS patients during an MS relapse and in a stable phase and from 18 healthy controls (HC). DNA belonging to Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). Human cytomegalovirus (HCMV), Epstein-Barr virus (EBV) and Human Herpes virus 6 (HHV-6) has been searched by specific nested polymerase chain reaction (n-PCR). EBV and HHV6 DNA has been detected with high frequency in acute and stable MS and in healthy controls without significant differences. HCMV DNA was observed both in acute and stable MS but not in HC, and, more interestingly, HSV-1 DNA was only found in 13% of acute MS, while both stable MS and healthy controls were negative. On the basis of these results we focused on HSV-1, and to confirm them and to demonstrate that HSV-1 is actively replicating in MS patients during clinical relapse, we searched both messenger RNA (mRNA) and DNA of HSV-1 in the PBMCs of 15 acute MS patients and 15 healthy controls. We found HSV-1 mRNA and DNA in a significant number of acute MS patients but not in the control group. On the whole these data indicate that HSV-1 reactivate in the peripheral blood of MS patients during clinical acute attack and probably play a role in the triggering of MS relapses.

KW - Clinical relapse

KW - DNA

KW - Herpes simplex type 1 and 2

KW - Herpesviruses

KW - Messenger RNA

KW - Multiple sclerosis

UR - http://www.scopus.com/inward/record.url?scp=0034040702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034040702&partnerID=8YFLogxK

M3 - Article

C2 - 10871797

AN - SCOPUS:0034040702

VL - 6

JO - Journal of NeuroVirology

JF - Journal of NeuroVirology

SN - 1355-0284

IS - SUPPL. 2

ER -