Molecular genetic changes in a series of neuroepithelial tumors of childhood

Alessia Di Sapio, Isabella Morra, Luca Pradotto, Marilena Guido, Davide Schiffer, Alessandro Mauro

Research output: Contribution to journalArticle

Abstract

Amplification of the EGFR, mdm2, CDK4 and PDGFR A genes has been widely demonstrated in adult malignant gliomas, almost exclusively glioblastomas. To determine the role of these mutational events in pediatric astrocytic gliomas we investigated the presence of EGFR, mdm2, CDK4 and PDGFR A gene amplification in 38 childhood brain tumor biopsies, including 24 low-grade astrocytomas and 14 malignant tumors. We used differential PCR assay on DNA extracted either from paraffin embedded or frozen tissues. EGFR gene amplification was detected in 4 out of 14 malignant tumors; no low-grade astrocytoma showed amplification. Tumors with EGFR gene amplification were negative for the presence of p53 mutations, as observed in a previous study. One glioblastoma showed PDGFR A amplification, while no amplifications were observed for mdm2 and CDK4 genes. These data are in line with those obtained from studies on gliomas of adults and suggest the existence of two different subsets of malignant gliomas also in pediatric brain tumors: one carrying EGFR gene amplification, the other showing p53 mutations.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalJournal of Neuro-Oncology
Volume59
Issue number2
DOIs
Publication statusPublished - Sep 2002

Keywords

  • CDK4
  • Childhood brain tumor
  • EGFR
  • Gene amplification
  • mdm2
  • p53 mutation
  • PDGFR A

ASJC Scopus subject areas

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neuroscience(all)

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