Molecular heterogeneity of blaVIM-2-containing integrons from Pseudomonas aeruginosa plasmids encoding the VIM-2 metallo-β-lactamase

Lucia Pallecchi, Maria Letizia Riccio, Jean Denis Docquier, Roberta Fontana, Gian Maria Rossolini

Research output: Contribution to journalArticlepeer-review

Abstract

A blaVIM-2 metallo-β-lactamase determinant, identical to that previously identified in Pseudomonas aeruginosa COL-1 isolate from a French hospital, was detected on a 28-kb plasmid carried by a nosocomial isolate of P. aeruginosa from Verona, Italy. In this plasmid the blaVIM-2 determinant was inserted into a class 1 integron of original structure, named In72, that contains a partially deleted intI1 integrase gene and two gene cassettes. The first cassette carries an aacA4 aminoglycoside acetyl transferase determinant. The second cassette carries a blaVIM-2 determinant followed by a partially deleted attC site. The structure of In72 was notably different from that of In56, the blaVIM-2-containing integron found in the COL-1 isolate, revealing the existence of molecular heterogeneity among blaVIM-2-containing integrons in clinical isolates of P. aeruginosa from Europe.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalFEMS Microbiology Letters
Volume195
Issue number2
DOIs
Publication statusPublished - Feb 20 2001

Keywords

  • Metallo-β-lactamase
  • Plasmid
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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