TY - JOUR
T1 - Molecular heterogeneity of blaVIM-2-containing integrons from Pseudomonas aeruginosa plasmids encoding the VIM-2 metallo-β-lactamase
AU - Pallecchi, Lucia
AU - Riccio, Maria Letizia
AU - Docquier, Jean Denis
AU - Fontana, Roberta
AU - Rossolini, Gian Maria
PY - 2001/2/20
Y1 - 2001/2/20
N2 - A blaVIM-2 metallo-β-lactamase determinant, identical to that previously identified in Pseudomonas aeruginosa COL-1 isolate from a French hospital, was detected on a 28-kb plasmid carried by a nosocomial isolate of P. aeruginosa from Verona, Italy. In this plasmid the blaVIM-2 determinant was inserted into a class 1 integron of original structure, named In72, that contains a partially deleted intI1 integrase gene and two gene cassettes. The first cassette carries an aacA4 aminoglycoside acetyl transferase determinant. The second cassette carries a blaVIM-2 determinant followed by a partially deleted attC site. The structure of In72 was notably different from that of In56, the blaVIM-2-containing integron found in the COL-1 isolate, revealing the existence of molecular heterogeneity among blaVIM-2-containing integrons in clinical isolates of P. aeruginosa from Europe.
AB - A blaVIM-2 metallo-β-lactamase determinant, identical to that previously identified in Pseudomonas aeruginosa COL-1 isolate from a French hospital, was detected on a 28-kb plasmid carried by a nosocomial isolate of P. aeruginosa from Verona, Italy. In this plasmid the blaVIM-2 determinant was inserted into a class 1 integron of original structure, named In72, that contains a partially deleted intI1 integrase gene and two gene cassettes. The first cassette carries an aacA4 aminoglycoside acetyl transferase determinant. The second cassette carries a blaVIM-2 determinant followed by a partially deleted attC site. The structure of In72 was notably different from that of In56, the blaVIM-2-containing integron found in the COL-1 isolate, revealing the existence of molecular heterogeneity among blaVIM-2-containing integrons in clinical isolates of P. aeruginosa from Europe.
KW - Metallo-β-lactamase
KW - Plasmid
KW - Pseudomonas aeruginosa
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U2 - 10.1016/S0378-1097(01)00002-7
DO - 10.1016/S0378-1097(01)00002-7
M3 - Article
C2 - 11179643
AN - SCOPUS:0035916418
VL - 195
SP - 145
EP - 150
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -