Molecular heterogeneity of regulatory elements of the mouse GATA-1 gene.

A. Ronchi, M. Cirò, L. Cairns, L. Basilico, P. Corbella, P. Ricciardi-Castagnoli, M. Cross, J. Ghysdael, S. Ottolenghi

Research output: Contribution to journalArticle

Abstract

The GATA-1 gene encodes a transcription factor expressed in early multipotent haemopoietic progenitors, in more mature cells of the erythroid, megakaryocytic and other lineages, but not in late myeloid precursors; its function is essential for the normal development of the erythroid and megakaryocytic system. To define regulatory elements of the mouse GATA-1 gene, we mapped DNaseI-hypersensitive sites in nuclei of erythroid and haemopoietic progenitor cells. Five sites were detected. The two upstream sites, site 1 and site 2, represent a new and a previously defined erythroid enhancer respectively. The site 1 enhancer activity depends both on a GATA-binding site (also footprinted in vivo) and on several sites capable of binding relatively ubiquitous factors. A DNA fragment encompassing site 1, placed upstream of a GATA-1 minimal promoter, is able to drive expression of a simian virus 40 (SV40) T-antigen in the yolk sac, but not bone-marrow cells, obtained from mice transgenic for this construct, allowing in vitro establishment of immortalized yolk-sac cells. A similar construct including site 2, instead of site 1, and previously shown to be able to immortalize adult marrow cells is not significantly active in yolk-sac cells. Sites 4 and 5, located in the first large intron, have no enhancer activity; they include a long array of potential Ets-binding sites. MnlI restriction sites, overlapping some of the Ets sites, are highly accessible, in intact nuclei, to MnlI. Although these sites are present in all GATA-1-expressing cells studied, they are the only strong sites detectable in FDCP-mix multipotent progenitor cells, most of which do not yet express GATA-1. The data indicate that appropriate GATA-1 regulation may require the co-operation of different regulatory elements acting at different stages of development and cell differentiation.

Original languageEnglish
Pages (from-to)245-258
Number of pages14
JournalGenes and function
Volume1
Issue number4
Publication statusPublished - Nov 1997

Fingerprint

Yolk Sac
Binding Sites
Genes
Erythroid Precursor Cells
Erythroid Cells
Simian virus 40
Viral Tumor Antigens
Bone Marrow Cells
Introns
Transgenic Mice
Cell Differentiation
Transcription Factors
Stem Cells
Bone Marrow
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Ronchi, A., Cirò, M., Cairns, L., Basilico, L., Corbella, P., Ricciardi-Castagnoli, P., ... Ottolenghi, S. (1997). Molecular heterogeneity of regulatory elements of the mouse GATA-1 gene. Genes and function, 1(4), 245-258.

Molecular heterogeneity of regulatory elements of the mouse GATA-1 gene. / Ronchi, A.; Cirò, M.; Cairns, L.; Basilico, L.; Corbella, P.; Ricciardi-Castagnoli, P.; Cross, M.; Ghysdael, J.; Ottolenghi, S.

In: Genes and function, Vol. 1, No. 4, 11.1997, p. 245-258.

Research output: Contribution to journalArticle

Ronchi, A, Cirò, M, Cairns, L, Basilico, L, Corbella, P, Ricciardi-Castagnoli, P, Cross, M, Ghysdael, J & Ottolenghi, S 1997, 'Molecular heterogeneity of regulatory elements of the mouse GATA-1 gene.', Genes and function, vol. 1, no. 4, pp. 245-258.
Ronchi A, Cirò M, Cairns L, Basilico L, Corbella P, Ricciardi-Castagnoli P et al. Molecular heterogeneity of regulatory elements of the mouse GATA-1 gene. Genes and function. 1997 Nov;1(4):245-258.
Ronchi, A. ; Cirò, M. ; Cairns, L. ; Basilico, L. ; Corbella, P. ; Ricciardi-Castagnoli, P. ; Cross, M. ; Ghysdael, J. ; Ottolenghi, S. / Molecular heterogeneity of regulatory elements of the mouse GATA-1 gene. In: Genes and function. 1997 ; Vol. 1, No. 4. pp. 245-258.
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