Molecular immune signatures of HIV-1 vaccines in human PBMCs

Alessandro Monaco, Francesco M. Marincola, Marianna Sabatino, Zoltan Pos, Maria Lina Tornesello, David F. Stroncek, Ena Wang, George K. Lewis, Franco M. Buonaguro, Luigi Buonaguro

Research output: Contribution to journalArticle

Abstract

The global transcriptional profile of peripheral blood mononuclear cells (PBMCs) stimulated with HIV candidate vaccine (virus-like particles, VLPs) has been evaluated in HIV-infected patients with low/high viral load compared to healthy volunteers. Baseline activation of chemokine production was observed in PBMC from HIV-infected patients and innate immune stimulation with HIV-VLPs was not blunted. The immune profile among HIV-infected patients was found to be qualitatively similar but quantitatively extremely variable. This diversity was independent of viral load and it might be dependent on individual immunogenetic traits or concurrent immunological status. This ex vivo screening strategy represents an efficient tool for guiding modifications/optimizations of vaccination strategies and understanding failures in individuals enrolled in clinical trials.

Original languageEnglish
Pages (from-to)3004-3008
Number of pages5
JournalFEBS Letters
Volume583
Issue number18
DOIs
Publication statusPublished - Sep 17 2009

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Keywords

  • HIV-1
  • Immunogenomics
  • Peripheral blood mononuclear cell
  • Vaccine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Monaco, A., Marincola, F. M., Sabatino, M., Pos, Z., Tornesello, M. L., Stroncek, D. F., Wang, E., Lewis, G. K., Buonaguro, F. M., & Buonaguro, L. (2009). Molecular immune signatures of HIV-1 vaccines in human PBMCs. FEBS Letters, 583(18), 3004-3008. https://doi.org/10.1016/j.febslet.2009.07.060