Molecular investigation of the cytokines produced by normal and malignant B lymphocytes

Marina Schena, Gianluca Gaidano, Daniela Gottardi, Fabio Malavasi, Lars Gunnar Larsson, Kenneth Nilsson, Federico Caligaris-Cappio

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Abstract

Different normal and malignant human B-cell populations were studied with a twofold aim: to define which cytokines are produced in vivo, and to assess the relationship between cytokine production and kinetic state. To analyse normal B-cells representative of different stages of activation and proliferation in vivo, we purified germinal centre (GC)-B blasts and mantle B (M-B) cells from tonsils. To compare malignant B lymphocytes with their closest normal equivalent cells, we separated malignant CD5+B lymphocytes from the peripheral blood of patients with B-chronic lymphocytic leukemia (B-CLL) and normal CD5+B lymphocytes from cord blood. The expression of interleukins (IL) IL-1α, IL-1β, tumour necrosis factor α (TNF-α), transforming growth factor β (TGF-β), IL-2, IL-4, and IL-6 genes was analysed using Northern and Western blotting techniques. TNF-α mRNA is produced by resting (M-B) and actively proliferating (GC-B) normal B lymphocytes. TGF-β mRNA is present at high levels in resting normal M-B cells, while the transcript levels are lower in proliferating GC-B and in activated CD5+B lymphocytes. IL-2 production is limited to the actively proliferating GC-B blasts, IL-1β and IL-6 to resting M-B cells. The cytokine production profile of CD5+ malignant B-CLL cells differs from that of their putative normal counterparts and is more like the profile of M-B cells, since B-CLL cells produce IL-1β, TNF-α, TGF-β, and IL-6. These observations lead to the following conclusions: among normal B lymphocyte populations, resting M-B lymphocytes are the most active cytokine producers, and B-CLL malignant B cells reflect the production pattern of normal resting B lymphocytes.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalLeukemia
Volume6
Issue number2
Publication statusPublished - Feb 1992

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B-Lymphocytes
Cytokines
Germinal Center
B-Cell Chronic Lymphocytic Leukemia
Interleukin-1
Interleukin-6
Interleukin-2
B-Cell Leukemia
Messenger RNA
Interleukins
Palatine Tonsil
Transforming Growth Factors
Fetal Blood
Interleukin-4
Northern Blotting
Population
Tumor Necrosis Factor-alpha
Western Blotting

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Schena, M., Gaidano, G., Gottardi, D., Malavasi, F., Larsson, L. G., Nilsson, K., & Caligaris-Cappio, F. (1992). Molecular investigation of the cytokines produced by normal and malignant B lymphocytes. Leukemia, 6(2), 120-125.

Molecular investigation of the cytokines produced by normal and malignant B lymphocytes. / Schena, Marina; Gaidano, Gianluca; Gottardi, Daniela; Malavasi, Fabio; Larsson, Lars Gunnar; Nilsson, Kenneth; Caligaris-Cappio, Federico.

In: Leukemia, Vol. 6, No. 2, 02.1992, p. 120-125.

Research output: Contribution to journalArticle

Schena, M, Gaidano, G, Gottardi, D, Malavasi, F, Larsson, LG, Nilsson, K & Caligaris-Cappio, F 1992, 'Molecular investigation of the cytokines produced by normal and malignant B lymphocytes', Leukemia, vol. 6, no. 2, pp. 120-125.
Schena M, Gaidano G, Gottardi D, Malavasi F, Larsson LG, Nilsson K et al. Molecular investigation of the cytokines produced by normal and malignant B lymphocytes. Leukemia. 1992 Feb;6(2):120-125.
Schena, Marina ; Gaidano, Gianluca ; Gottardi, Daniela ; Malavasi, Fabio ; Larsson, Lars Gunnar ; Nilsson, Kenneth ; Caligaris-Cappio, Federico. / Molecular investigation of the cytokines produced by normal and malignant B lymphocytes. In: Leukemia. 1992 ; Vol. 6, No. 2. pp. 120-125.
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