Abstract
Objective: Insulin resistance is a strong biological marker of both obesity and type 2 diabetes. Abnormal fat deposition within skeletal muscle has been identified as a mechanism of obesity-associated insulin resistance. Biliopancreatic diversion (BPD), inducing a massive lipid malabsorption, leads to a reversion of type 2 diabetes. To elucidate the mechanisms of diabetes reversibility, the expression of genes involved in glucose and free fatty acids (FFAs) metabolism was investigated in skeletal muscle biopsies from obese, type 2 diabetic subjects. Peripheral insulin sensitivity and insulin secretion was also measured. Subjects: Eight Caucasian obese diabetic patients (BMI 52.1±1.85 kg/m2) were studied before and 3 years after BPD. Measurements: The mRNA levels were estimated by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR), insulin sensitivity by the euglycemic-hyperinsulinemic clamp and insulin secretion using a model describing the relationship between insulin secretion and glucose concentration. Results: Whole-body glucose uptake (M), normalized by fat-free mass, significantly increased in post-obese subjects (P
Original language | English |
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Pages (from-to) | 1429-1436 |
Number of pages | 8 |
Journal | International Journal of Obesity |
Volume | 31 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sep 2007 |
Keywords
- Biliopancreatic diversion
- Gene expression
- Insulin resistance
- Morbid obesity
- Type 2 diabetes
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Public Health, Environmental and Occupational Health
- Endocrinology
- Food Science
- Endocrinology, Diabetes and Metabolism