Molecular monitoring of organ recipients from cancer-affected donors by detection of circulating tumor cells

E. Gabusi, B. Corti, A. D'Errico, L. Ridolfi, G. Ercolani, S. Venettoni, A. N. Costa, W. F. Grigioni

Research output: Contribution to journalArticlepeer-review

Abstract

We have initiated regular molecular monitoring based on nested RT-PCR detection of circulating tumor cells for monitoring recipients of organs from cancer-affected donors in Italy (in the context of a "Donation Safety and Donated Organ Quality" project organized by the Centro Nazionale Trapianti). Five patients are being monitored. For two patients who each received a kidney from a single donor with prostate adenocarcinoma, RT-PCR was performed using PSA mRNA. For three recipients of organs (two livers and one kidney) from donors with renal cell carcinoma, RT-PCR was performed using cytokeratine 18 and 19 mRNA. Blood samples from five healthy subjects were used as negative controls. After a median monitoring time of 26 months (range 8 to 32), none of the regular 3-month assays has tested positive. This pilot study suggests that detection of circulating tumor cells by nested RT-PCR may provide a feasible molecular monitoring, which might assist decision making regarding other forms of clinical surveillance.

Original languageEnglish
Pages (from-to)1344-1347
Number of pages4
JournalTransplantation Proceedings
Volume36
Issue number5
DOIs
Publication statusPublished - Jun 2004

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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