Molecular profile of a human monoclonal antibody fab fragment specific for epstein-barr virus gp350/220 antigen

Francesca Bugli, Raiza Bastidas, Dennis R. Burton, R. Anthony Williamson, Massimo Clementi, Roberto Burioni

Research output: Contribution to journalArticle

Abstract

Experimental evidence indicates Epstein Barr vitus (EBV) envelope glycoprotein gp350/220 elicits a potent virus neutralizing response in the infected human host that may play an important role in restricting viral pathogenesis. In this study, we report the molecular cloning in combinatorial phage display vectors, of the IgG1 repertoire of an individual naturally infected with EBV, and describe the recovery and characterization of a monoclonal antibody recognizing gp350/220. A detailed understanding of the human antibody response in EBV infection will identify antibodies of potential use in antiviral prophylaxis and will advance the production of more effective vaccine candidates.

Original languageEnglish
Pages (from-to)362-367
Number of pages6
JournalHuman Immunology
Volume62
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Combinatorial library
  • Epstein-Barr virus
  • Human monoclonal antibodies
  • Phage display

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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