Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes

Cinzia Di Pietro, Marilena Vento, Maria Rosa Guglielmino, Placido Borz, Manuela Santonocito, Marco Ragusa, Davide Barbagallo, Laura Rita Duro, Alessandra Majorana, Adele De Palma, Maria Rita Garofalo, Elisa Minutolo, Paolo Scollo, Michele Purrello

Research output: Contribution to journalArticlepeer-review

Abstract

To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed through real-time reverse transcriptase-polymerase chain reaction eight genes encoding critically important proteins for embryo development and compared this partial transcriptome with that of freshly collected gametes isolated from the same women. The comparison of the molecular profiles demonstrated that our vitrification protocol does not alter the biomolecular quality of oocytes: in fact, between the two groups we found the absence of statistically significant variations. Accordingly, this cryopreservation technique might be helpful in preserving women's fertility.

Original languageEnglish
Pages (from-to)2804-2807
Number of pages4
JournalFertility and Sterility
Volume94
Issue number7
DOIs
Publication statusPublished - Dec 2010

Keywords

  • gene expression
  • Human oocytes
  • molecular markers
  • real time RT-PCR
  • vitrification

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

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