Molecular signature of disease onset in Granulin mutation carriers: A gene expression analysis study

Elena Milanesi, Cristian Bonvicini, Antonella Alberici, Andrea Pilotto, Nadia Cattane, Enrico Premi, Stefano Gazzina, Silvana Archetti, Roberto Gasparotti, Vanessa Cancelli, Massimo Gennarelli, Alessandro Padovani, Barbara Borroni

Research output: Contribution to journalArticle

Abstract

Mutations within Granulin (GRN) gene are causative of autosomal dominant frontotemporal lobar degeneration (FTLD). Though GRN mutations are inherited at birth, the disease onset usually occurs in the sixth decade of life. The objective of this study was to identify new genetic pathways linked to inherited GRN disease and involved in the shift from asymptomatic to symptomatic stages. Microarray gene expression analysis on leukocytes was carried out on 15 patients carrying GRN T272SfsX10 mutation, and their asymptomatic siblings with (n = 14) or without (n = 11) GRN mutation. The results were then validated by real-time polymerase chain reaction, and compared with those obtained in a cohort of FTLD without GRN mutation (n = 16). The association between candidate genes and damage of specific brain areas was investigated by voxel-based morphometry on magnetic resonance imaging scans (family-wise error-corrected). Leukocytes mRNA levels of TMEM40 and LY6G6F and other genes mainly involved in inflammation were significantly higher in patients carrying GRN mutations compared with asymptomatic carriers and other FTLD. The higher the levels of TMEM40 the greater is the damage of parietal lobule; the higher the LY6G6F gene expression the greater is the atrophy in superior frontal gyrus. Enhanced inflammation associated with the onset of GRN disease might be either related to disease pathogenetic mechanism leading to neurodegeneration or to a compensatory pathway that counteracts disease progression. The identification of specific molecular targets of GRN-FTLD disease is essential when considering future disease-modifying therapies.

Original languageEnglish
Pages (from-to)1837-1845
Number of pages9
JournalNeurobiology of Aging
Volume34
Issue number7
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Frontotemporal dementia
  • Frontotemporal lobar degeneration
  • Gene expression
  • Granulin
  • Inflammation
  • Mutations
  • Progranulin

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Ageing
  • Developmental Biology
  • Geriatrics and Gerontology

Fingerprint Dive into the research topics of 'Molecular signature of disease onset in Granulin mutation carriers: A gene expression analysis study'. Together they form a unique fingerprint.

  • Cite this

    Milanesi, E., Bonvicini, C., Alberici, A., Pilotto, A., Cattane, N., Premi, E., Gazzina, S., Archetti, S., Gasparotti, R., Cancelli, V., Gennarelli, M., Padovani, A., & Borroni, B. (2013). Molecular signature of disease onset in Granulin mutation carriers: A gene expression analysis study. Neurobiology of Aging, 34(7), 1837-1845. https://doi.org/10.1016/j.neurobiolaging.2012.11.016