Molecular strategies for detecting chromosomal translocations in soft tissue tumors (review)

Margherita Cerrone, Monica Cantile, Francesca Collina, Laura Marra, Giuseppina Liguori, Renato Franco, Annarosaria De Chiara, Gerardo Botti

Research output: Contribution to journalArticle

Abstract

Approximately one third of soft tissue tumors are characterized by chromosomal aberrations, in particular, translocations and amplifications, which appear to be highly specific. The identification of fusion transcripts not only supports the diagnosis, but provides the basis for the development of novel therapeutic strategies aimed at blocking the aberrant activity of chimeric proteins. Molecular biology, and in particular, cytogenetic and qualitative and quantitative polymerase chain reaction technologies, allow with high efficiency and specificity, the determination of specific fusion transcripts resulting from chromosomal translocations, as well as the analysis of gene amplifications. In this review, various molecular techniques that allow the identification of translocations and consequent fusion transcripts generated are discussed in the broad spectrum of soft tissue tumors.

Original languageEnglish
Pages (from-to)1379-1391
Number of pages13
JournalInternational Journal of Molecular Medicine
Volume33
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • Chromosomal translocations
  • Molecular analyses
  • Soft tissue tumors

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Fingerprint Dive into the research topics of 'Molecular strategies for detecting chromosomal translocations in soft tissue tumors (review)'. Together they form a unique fingerprint.

  • Cite this