TY - JOUR
T1 - Molecular studies of cerebrospinal fluid in human immunodeficiency virus type 1-associated opportunistic central nervous system diseases - An update
AU - Cinque, Paola
AU - Bossolasco, Simona
AU - Bestetti, Arabella
AU - Sala, Serena
AU - Pierotti, Chiara
AU - Lazzarin, Adriano
PY - 2002
Y1 - 2002
N2 - Although the incidence of opportunistic central nervous system (CNS) diseases has markedly declined in developed countries following the advent of highly active antiretroviral therapies (HAARTs), they still represent a major diagnostic and therapeutic challenge over the world. The application of nucleic acid amplification techniques to the study of cerebrospinal fluid (CSF) has contributed substantially to their diagnosis. The detection of specific microbial genomes in the CSF is now the preferred test for some CNS opportunistic diseases, such as progressive multifocal leukoencephalopathy or cytomegalovirus encephalitis. More recent developments of these techniques are the quantitative amplification techniques and postamplification studies. Quantification of nucleic acids in CSF is an important aid both at the time of diagnosis, for the interpretation of positive findings, and during patient follow-up. Postamplification analyses can provide important information with regard to clinical patient management, e.g., detection of genotypic resistance to antimicrobial drugs, and in the attempt to elucidate disease epidemiology and pathogenesis.
AB - Although the incidence of opportunistic central nervous system (CNS) diseases has markedly declined in developed countries following the advent of highly active antiretroviral therapies (HAARTs), they still represent a major diagnostic and therapeutic challenge over the world. The application of nucleic acid amplification techniques to the study of cerebrospinal fluid (CSF) has contributed substantially to their diagnosis. The detection of specific microbial genomes in the CSF is now the preferred test for some CNS opportunistic diseases, such as progressive multifocal leukoencephalopathy or cytomegalovirus encephalitis. More recent developments of these techniques are the quantitative amplification techniques and postamplification studies. Quantification of nucleic acids in CSF is an important aid both at the time of diagnosis, for the interpretation of positive findings, and during patient follow-up. Postamplification analyses can provide important information with regard to clinical patient management, e.g., detection of genotypic resistance to antimicrobial drugs, and in the attempt to elucidate disease epidemiology and pathogenesis.
KW - Cerebrospinal fluid
KW - HIV
KW - Nucleic acid amplification
KW - Opportunistic infections
KW - Polymerase chain reaction
KW - Progressive multifocal leukoencephalopathy
UR - http://www.scopus.com/inward/record.url?scp=0036948152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036948152&partnerID=8YFLogxK
M3 - Article
C2 - 12491163
AN - SCOPUS:0036948152
VL - 8
SP - 122
EP - 128
JO - Journal of NeuroVirology
JF - Journal of NeuroVirology
SN - 1355-0284
IS - SUPPL. 2
ER -