Molecular study of human herpesvirus 6 and 8 involvement in coronary atherosclerosis and coronary instability

Marco Magnoni, Mauro Malnati, Nicole Cristell, Stefano Coli, Domenico Russo, Giacomo Ruotolo, Domenico Cianflone, Ottavio Alfieri, Paolo Lusso, Attilio Maseri

Research output: Contribution to journalArticle

Abstract

Several lines of evidence suggest the involvement of infectious agents in the pathogenesis of atherosclerosis. Furthermore, a correlation between infection-driven inflammatory burden and acute manifestation of coronary artery disease has been hypothesized. The aim of this work was to assess whether human herpesvirus (HHV)-6 and HHV-8, two DNA viruses with a distinct tropism for endothelium and lymphocytes, may be associated with coronary instability. An age- and gender-matched cross-sectional study was undertaken in 70 patients with testing of plasma HHV-6 and HHV-8 DNA load in different cardiovascular clinical settings: 29 patients with acute myocardial infarction, 21 patients with stable coronary artery disease, and 20 patients without coronary and carotid artery atherosclerosis subjected to cardiac valve replacement. In all patients, HHV-6 and HHV-8 plasma DNA was tested by using highly sensitive, calibrated quantitative real-time PCR assays which employ a synthetic DNA calibrator to adjust for DNA extraction and amplification efficiency. HHV-8 viremia was undetectable in all three groups. HHV-6 viremia was detected in a substantial fraction of the samples examined (18.6%) without significant differences among the three groups (ST segment elevation myocardial infarction: 17.2%; stable coronary artery disease: 14.3%; patients without coronary and carotid artery atherosclerosis: 25%). Furthermore, no significant differences in plasma HHV-6 load were observed amongst the three groups of patients. These findings indicate that coronary instability is not associated specifically with active HHV-6 or HHV-8 infection. However, an unusually high rate of active HHV-6 infection was documented among patients without atherosclerosis admitted to hospital with cardiac disease.

Original languageEnglish
Pages (from-to)1961-1966
Number of pages6
JournalJournal of Medical Virology
Volume84
Issue number12
DOIs
Publication statusPublished - Dec 2012

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Human Herpesvirus 6
Human Herpesvirus 8
Coronary Artery Disease
Herpesviridae Infections
Carotid Artery Diseases
Viremia
DNA
Carotid Arteries
Atherosclerosis
Coronary Vessels
Tropism
DNA Viruses
Heart Valves
Endothelium
Real-Time Polymerase Chain Reaction
Heart Diseases
Cross-Sectional Studies
Myocardial Infarction
Lymphocytes

Keywords

  • Acute coronary syndrome
  • Atherosclerosis
  • Infection
  • Virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Molecular study of human herpesvirus 6 and 8 involvement in coronary atherosclerosis and coronary instability. / Magnoni, Marco; Malnati, Mauro; Cristell, Nicole; Coli, Stefano; Russo, Domenico; Ruotolo, Giacomo; Cianflone, Domenico; Alfieri, Ottavio; Lusso, Paolo; Maseri, Attilio.

In: Journal of Medical Virology, Vol. 84, No. 12, 12.2012, p. 1961-1966.

Research output: Contribution to journalArticle

Magnoni, Marco ; Malnati, Mauro ; Cristell, Nicole ; Coli, Stefano ; Russo, Domenico ; Ruotolo, Giacomo ; Cianflone, Domenico ; Alfieri, Ottavio ; Lusso, Paolo ; Maseri, Attilio. / Molecular study of human herpesvirus 6 and 8 involvement in coronary atherosclerosis and coronary instability. In: Journal of Medical Virology. 2012 ; Vol. 84, No. 12. pp. 1961-1966.
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