TY - JOUR
T1 - Molecular Transmission Dynamics of Primary HIV Infections in Lazio Region, Years 2013-2020
AU - Fabeni, Lavinia
AU - Rozera, Gabriella
AU - Berno, Giulia
AU - Giombini, Emanuela
AU - Gori, Caterina
AU - Orchi, Nicoletta
AU - De Carli, Gabriella
AU - Pittalis, Silvia
AU - Puro, Vincenzo
AU - Pinnetti, Carmela
AU - Mondi, Annalisa
AU - Camici, Marta
AU - Plazzi, Maria Maddalena
AU - Antinori, Andrea
AU - Capobianchi, Maria Rosaria
AU - Abbate, Isabella
PY - 2021/1/25
Y1 - 2021/1/25
N2 - Molecular investigation of primary HIV infections (PHI) is crucial to describe current dynamics of HIV transmission. Aim of the study was to investigate HIV transmission clusters (TC) in PHI referred during the years 2013-2020 to the National Institute for Infectious Diseases in Rome (INMI), that is the Lazio regional AIDS reference centre, and factors possibly associated with inclusion in TC. These were identified by phylogenetic analysis, based on population sequencing of pol; a more in depth analysis was performed on TC of B subtype, using ultra-deep sequencing (UDS) of env. Of 270 patients diagnosed with PHI during the study period, 229 were enrolled (median follow-up 168 (IQR 96-232) weeks). Median age: 39 (IQR 32-48) years; 94.8% males, 86.5% Italians, 83.4% MSM, 56.8% carrying HIV-1 subtype B. Of them, 92.6% started early treatment within a median of 4 (IQR 2-7) days after diagnosis; median time to sustained suppression was 20 (IQR 8-32) weeks. Twenty TC (median size 3, range 2-9 individuals), including 68 patients, were identified. A diagnosis prior to 2015 was the unique factor associated with inclusion in a TC. Added value of UDS was the identification of shared quasispecies components in transmission pairs within TC.
AB - Molecular investigation of primary HIV infections (PHI) is crucial to describe current dynamics of HIV transmission. Aim of the study was to investigate HIV transmission clusters (TC) in PHI referred during the years 2013-2020 to the National Institute for Infectious Diseases in Rome (INMI), that is the Lazio regional AIDS reference centre, and factors possibly associated with inclusion in TC. These were identified by phylogenetic analysis, based on population sequencing of pol; a more in depth analysis was performed on TC of B subtype, using ultra-deep sequencing (UDS) of env. Of 270 patients diagnosed with PHI during the study period, 229 were enrolled (median follow-up 168 (IQR 96-232) weeks). Median age: 39 (IQR 32-48) years; 94.8% males, 86.5% Italians, 83.4% MSM, 56.8% carrying HIV-1 subtype B. Of them, 92.6% started early treatment within a median of 4 (IQR 2-7) days after diagnosis; median time to sustained suppression was 20 (IQR 8-32) weeks. Twenty TC (median size 3, range 2-9 individuals), including 68 patients, were identified. A diagnosis prior to 2015 was the unique factor associated with inclusion in a TC. Added value of UDS was the identification of shared quasispecies components in transmission pairs within TC.
KW - HIV primary infection
KW - phylogenetic analysis
KW - spread and epidemiology
KW - ultra-deep sequencing
UR - http://www.scopus.com/inward/record.url?scp=85100657077&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85100657077&partnerID=8YFLogxK
U2 - 10.3390/v13020176
DO - 10.3390/v13020176
M3 - Article
C2 - 33503987
AN - SCOPUS:85100657077
VL - 13
SP - 1
EP - 13
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 2
M1 - 176
ER -