Molecular typing of burkholderia cepacia complex isolated from patients attending an Italian cystic fibrosis centre

Antonio Teri, Samantha Sottotetti, Arianna Biffi, Daniela Girelli, Monica D’Accico, Milena Arghittu, Carla Colombo, Fabiola Corti, Giovanna Pizzamiglio, Lisa Cariani

Research output: Contribution to journalArticlepeer-review

Abstract

Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). Bcc infection is often extremely difficult to treat due to its intrinsic resistance to multiple antibiotics. In addition, it seems to speed up the decline of lung function and is considered a contraindication for lung transplantation in CF. This study investigates the species of the Bcc strains recovered from chronically infected CF subjects by means of: isolation, identification methods and complete recA nucleotide sequences of 151 samples. Molecular typing showed that B. cenocepacia III is the dominant strain found in the group of subjects being treated at the Milan CF Centre (Italy) and that the infection is chronically maintained by the same species. Defining species by means of molecular analysis yields important information for the clinician in order to establish the most appropriate therapy and implement correct measures for prevention of transmission among CF subjects.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalNew Microbiologica
Volume41
Issue number2
Publication statusPublished - Apr 1 2018

Keywords

  • Burkholderia cenocepacia
  • Burkholderia cepacia complex
  • Cystic fibrosis
  • Genomovar
  • Molecular typing
  • RecA

ASJC Scopus subject areas

  • Microbiology (medical)

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