Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats

S. Cresti, B. Posteraro, M. Sanguinetti, P. Guglielmetti, G. M. Rossolini, G. Morace, G. Fadda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Random amplification of polymorphic DNA (RAPD) using an arbitrary oligonucleotide primer (5′-CGGTGCGACG) and analysis of restriction fragment length polymorphism of ribosomal DNA (rDNA-RFLP) after digestion of genomic DNA with restriction endonuclease EcoRI were investigated as tools for genotypic delineation beyond the species level of 91 Candida clinical isolates and four reference strains including 33 Candida albicans, 19 Candida tropicalis, 22 Candida krusei and 21 Candida (Torulopsis) glabrata. Results indicated that both techniques can be useful for typing isolates of the above species, although showing a variable discriminative potential with different species. As compared to RAPD fingerprinting, the discriminative potential of rDNA-RFLP appeared to be highest for C. albicans and lowest for C. glabrata, being overall similar for C. krusei and identical for C. tropicalis. A comparative analysis of the results obtained with the two typing techniques showed that, except for C. tropicalis, they were able to provide non-redundant information, and that their use in combination could enhance the discriminative potential for delineation among C. glabrata and C. krusei isolates.

Original languageEnglish
Pages (from-to)41-52
Number of pages12
JournalNew Microbiologica
Volume22
Issue number1
Publication statusPublished - Jan 1999

Fingerprint

Candida tropicalis
Molecular Typing
Ribosomal DNA
Candida
Restriction Fragment Length Polymorphisms
Candida glabrata
Candida albicans
DNA
Deoxyribonuclease EcoRI
DNA Primers
DNA Fingerprinting
DNA Restriction Enzymes
Digestion

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Cresti, S., Posteraro, B., Sanguinetti, M., Guglielmetti, P., Rossolini, G. M., Morace, G., & Fadda, G. (1999). Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats. New Microbiologica, 22(1), 41-52.

Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats. / Cresti, S.; Posteraro, B.; Sanguinetti, M.; Guglielmetti, P.; Rossolini, G. M.; Morace, G.; Fadda, G.

In: New Microbiologica, Vol. 22, No. 1, 01.1999, p. 41-52.

Research output: Contribution to journalArticle

Cresti, S, Posteraro, B, Sanguinetti, M, Guglielmetti, P, Rossolini, GM, Morace, G & Fadda, G 1999, 'Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats', New Microbiologica, vol. 22, no. 1, pp. 41-52.
Cresti, S. ; Posteraro, B. ; Sanguinetti, M. ; Guglielmetti, P. ; Rossolini, G. M. ; Morace, G. ; Fadda, G. / Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restriction fragment length polymorphism of ribosomal DNA repeats. In: New Microbiologica. 1999 ; Vol. 22, No. 1. pp. 41-52.
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