Molecular typing of staphylococcus aureus isolates from an intensive care unit

G. Zanelli, S. Pollini, A. Sansoni, S. Cresti, E. Pilli, G. M. Rossolini, C. Cellesi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Seventeen S. aureus clinical isolates, collected from an Intensive Care Unit (ICU) during a seven-month period were analyzed to investigate their antimicrobial susceptibility and clonal diversity. Eleven isolates (65%) were found to be resistant to methicillin (MRSA). Pulsed-field gel electrophoresis (PFGE) profiles of genomic DNAs, and analysis of the polymorphisms of the variable regions of the protein A (spa) and coagulase (coa) genes revealed a lower clonal heterogeneity among MRSA than among methicillin-susceptible isolates (MSSA). Two of the MRSA clones were repeatedly isolated in different patients, within a variable period of time, suggesting the presence in the ward of a resident, endemic and multi-drug resistant MRSA population. Our results also emphasize the lower discriminatory power of spa and coa typing compared with PFGE typing.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalNew Microbiologica
Volume27
Issue number3
Publication statusPublished - Jul 2004

Fingerprint

Molecular Typing
Methicillin-Resistant Staphylococcus aureus
Intensive Care Units
Staphylococcus aureus
Coagulase
Pulsed Field Gel Electrophoresis
Methicillin Resistance
Methicillin
Staphylococcal Protein A
Clone Cells
DNA
Pharmaceutical Preparations
Population
Genes

Keywords

  • Intensive care unit
  • Molecular typing
  • S. aureus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Zanelli, G., Pollini, S., Sansoni, A., Cresti, S., Pilli, E., Rossolini, G. M., & Cellesi, C. (2004). Molecular typing of staphylococcus aureus isolates from an intensive care unit. New Microbiologica, 27(3), 293-299.

Molecular typing of staphylococcus aureus isolates from an intensive care unit. / Zanelli, G.; Pollini, S.; Sansoni, A.; Cresti, S.; Pilli, E.; Rossolini, G. M.; Cellesi, C.

In: New Microbiologica, Vol. 27, No. 3, 07.2004, p. 293-299.

Research output: Contribution to journalArticle

Zanelli, G, Pollini, S, Sansoni, A, Cresti, S, Pilli, E, Rossolini, GM & Cellesi, C 2004, 'Molecular typing of staphylococcus aureus isolates from an intensive care unit', New Microbiologica, vol. 27, no. 3, pp. 293-299.
Zanelli G, Pollini S, Sansoni A, Cresti S, Pilli E, Rossolini GM et al. Molecular typing of staphylococcus aureus isolates from an intensive care unit. New Microbiologica. 2004 Jul;27(3):293-299.
Zanelli, G. ; Pollini, S. ; Sansoni, A. ; Cresti, S. ; Pilli, E. ; Rossolini, G. M. ; Cellesi, C. / Molecular typing of staphylococcus aureus isolates from an intensive care unit. In: New Microbiologica. 2004 ; Vol. 27, No. 3. pp. 293-299.
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