Molecular typing of staphylococcus aureus isolates from an intensive care unit

G. Zanelli, S. Pollini, A. Sansoni, S. Cresti, E. Pilli, G. M. Rossolini, C. Cellesi

Research output: Contribution to journalArticle


Seventeen S. aureus clinical isolates, collected from an Intensive Care Unit (ICU) during a seven-month period were analyzed to investigate their antimicrobial susceptibility and clonal diversity. Eleven isolates (65%) were found to be resistant to methicillin (MRSA). Pulsed-field gel electrophoresis (PFGE) profiles of genomic DNAs, and analysis of the polymorphisms of the variable regions of the protein A (spa) and coagulase (coa) genes revealed a lower clonal heterogeneity among MRSA than among methicillin-susceptible isolates (MSSA). Two of the MRSA clones were repeatedly isolated in different patients, within a variable period of time, suggesting the presence in the ward of a resident, endemic and multi-drug resistant MRSA population. Our results also emphasize the lower discriminatory power of spa and coa typing compared with PFGE typing.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalNew Microbiologica
Issue number3
Publication statusPublished - Jul 2004



  • Intensive care unit
  • Molecular typing
  • S. aureus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Zanelli, G., Pollini, S., Sansoni, A., Cresti, S., Pilli, E., Rossolini, G. M., & Cellesi, C. (2004). Molecular typing of staphylococcus aureus isolates from an intensive care unit. New Microbiologica, 27(3), 293-299.