Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching

Florian Mueller, Tatiana S. Karpova, Davide Mazza, James G. McNally

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescence recovery after photobleaching (FRAP) has now become widely used to investigate nuclear protein binding to chromatin in live cells. FRAP can be applied qualitatively to assess if chromatin binding interactions are altered by various biological perturbations. It can also be applied semi-quantitatively to allow numerical comparisons between FRAP curves, and even fully quantitatively to yield estimates of in vivo diffusion constants and nuclear protein binding rates to chromatin. Here we describe how FRAP data should be collected and processed for these qualitative, semi-quantitative, and quantitative analyses.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages153-176
Number of pages24
Volume833
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume833
ISSN (Print)10643745

Keywords

  • Binding
  • Chromatin
  • FRAP
  • Microscopy
  • Modeling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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